Mouse TRAIL R2/DR5/TNFRSF10B Protein (TRL-MM1R2)

Mouse TRAIL R2, His Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.68 nM as determined in SPR assay (Biacore T200).

Mouse TRAIL R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Mouse TRAIL R2 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.68 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Asn53-Lys180

Catalog #TRL-MM1R2

Price

$223³⁰

Size

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Mouse TRAIL R2/DR5/TNFRSF10B Protein (TRL-MM1R2) Summary

Product Description

Recombinant Mouse TRAIL R2/DR5/TNFRSF10B Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asn53-Lys180.

Product Background

DR5, also called TRAIL R2, TRICK 2, TNFRSF10B, and MK is a type 1 TNF R superfamily, membrane protein which is a receptor for TRAIL (APO2 ligand). DR5 is a receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis.

Mouse TRAIL R2/DR5/TNFRSF10B Protein (TRL-MM1R2) Specifications/Details

Protein	TRAIL R2/DR5/TNFRSF10B
Synonyms	CD262; DR5; DR5TRICK2B; Fas-like protein; KILLER/DR5; TNFRSF10B; TRAIL R2; TRAIL receptor 2; TRAILR2; TRICK2; TRICK2A; TRICKB; ZTNFR9; KILLER
Accession	Q9QZM4-1
Species	Mouse
Biotinylated	no
Amino Acid Range	Asn53-Lys180
Molecular Weight	The protein has a predicted MW of 15.3 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 0.68 nM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Mouse TRAIL R2, His Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.68 nM as determined in SPR assay (Biacore T200).
	Mouse TRAIL R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Mouse TRAIL R2 is greater than 95% as determined by SEC-HPLC.