Biotinylated Cynomolgus CD3E/CD3 epsilon 1-27 Protein (CD3-CM2EDB)
Biotinylated Cynomolgus CD3E/CD3 epsilon 1-27 Protein (CD3-CM2EDB)
Biotinylated Cynomolgus CD3E/CD3 epsilon 1-27 Protein (CD3-CM2EDB)
  • Express System: HEK293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Anti-CD3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E 1-27, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Asp22-Thr48
Catalog #CD3-CM2EDB
Regular price $660.00
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Product Description

Recombinant Biotinylated Cynomolgus CD3E/CD3 epsilon 1-27 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Asp22-Thr48.

Product Background

CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

CD3E/CD3 epsilon 1-27

Synonyms

CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18

Accession

Species

Cynomolgus

Biotinylated

yes

Amino Acid Range

Asp22-Thr48

Molecular Weight

The protein has a predicted MW of 31.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Anti-CD3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E 1-27, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Anti-CD3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E 1-27, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA. Immobilized Anti-CD3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E 1-27, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA.
Biotinylated Cynomolgus CD3E 1-27 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Cynomolgus CD3E 1-27 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus CD3E 1-27 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Cynomolgus CD3E 1-27 is greater than 95% as determined by SEC-HPLC.

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