Name: Biotinylated Cynomolgus CD3E/CD3 epsilon 1-27 Protein (CD3-CM2EDB)
Brand: KactusBio
SKU: CD3-CM2EDB-100UG
Price: 660.0 USD
Availability: InStock

Biotinylated Cynomolgus CD3E/CD3 epsilon 1-27 Protein (CD3-CM2EDB)

Immobilized Anti-CD3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E 1-27, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA.

Biotinylated Cynomolgus CD3E 1-27 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Cynomolgus CD3E 1-27 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Anti-CD3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E 1-27, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Asp22-Thr48

Catalog #CD3-CM2EDB

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Cynomolgus CD3E/CD3 epsilon 1-27 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Asp22-Thr48.
Product Background	CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	CD3E/CD3 epsilon 1-27
Synonyms	CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18
Accession	Q95LI5.2
Species	Cynomolgus
Biotinylated	yes
Amino Acid Range	Asp22-Thr48
Molecular Weight	The protein has a predicted MW of 31.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Anti-CD3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E 1-27, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Anti-CD3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E 1-27, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA.
	Biotinylated Cynomolgus CD3E 1-27 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Cynomolgus CD3E 1-27 is greater than 95% as determined by SEC-HPLC.