Biotinylated Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) (CD3-CM201B)
Biotinylated Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) (CD3-CM201B)
Biotinylated Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) (CD3-CM201B)
Biotinylated Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) (CD3-CM201B)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Anti-CD3 Antibody, hFc Tag at 2ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E&CD3D, hFc Tag with the EC50 of 0.10ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln22-Asp117(CD3E) & Phe22-Ala105(CD3D)
Catalog #CD3-CM201B
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Product Description
 Recombinant Biotinylated Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the C-Terminus.; It contains Gln22-Asp117 (CD3E) and Phe22-Ala105 (CD3D).
Product Background
 T-cell surface glycoprotein CD3 epsilon&CD3 delta chain, also known as CD3E&CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Product Category
 Recombinant Protein
Target
 CD3E&CD3D/CD3 epsilon&CD3 delta
Synonyms
 CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon
Accession
Species
 Cynomolgus
Biotinylated
 yes
Amino Acid Range
 Gln22-Asp117(CD3E) & Phe22-Ala105(CD3D)
Molecular Weight
 The protein has a predicted MW of 36.9 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation,the protein migrates to 58-62 kDa and 44-46 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Anti-CD3 Antibody, hFc Tag at 2ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E&CD3D, hFc Tag with the EC50 of 0.10ug/ml determined by ELISA. See testing image for detail.
Product Tag
 C-hFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-CD3 Antibody, hFc Tag at 2ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E&CD3D, hFc Tag with the EC50 of 0.10ug/ml determined by ELISA.
Biotinylated Cynomolgus CD3E&CD3D on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus CD3E&CD3D was greater than 95% as determined by SEC-HPLC.

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