Biotinylated Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) (CD3-CM201B)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-CD3, hFc Tag Antibody at 5_g/ml (100_l/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E&CD3D, hFc Tag with the EC50 of 58.2ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln22-Asp117(CD3E) & Phe22-Ala105(CD3D)

Catalog: CD3-CM201B

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Product Description Recombinant Biotinylated Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) is expressed from Expi293 with hFc tag at the C-terminal.It contains Gln22 - Asp117(CD3E)&Phe22-Ala105(CD3D).
Product Category Recombinant Protein
Target CD3E&CD3D/CD3 epsilon&CD3 delta
Synonyms CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon
Gene/Protein ID Q95LI5.2(CD3E)&Q95LI8(CD3D)
Species Cynomolgus
Exact Sequence Gln22-Asp117(CD3E) & Phe22-Ala105(CD3D)
Molecular Weight The protein has a predicted MW of 36.9 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation,the protein migrates to 58-62 kDa and 44-46 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-CD3, hFc Tag Antibody at 5_g/ml (100_l/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E&CD3D, hFc Tag with the EC50 of 58.2ng/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per _g by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22_m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-CD3, hFc Tag Antibody at 5_g/ml (100_l/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E&CD3D, hFc Tag with the EC50 of 58.2ng/ml determined by ELISA.
The purity of Biotinylated Cynomolgus CD3E&CD3D was greater than 95% as determined by SEC-HPLC.
Biotinylated Cynomolgus CD3E&CD3D on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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