Biotinylated Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) (CD3-CM201B)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-CD3, hFc Tag Antibody at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E&CD3D, hFc Tag with the EC50 of 58.2ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln22-Asp117 (CD3E)&Phe22-Ala105 (CD3D)

Catalog: CD3-CM201B

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Product Description Recombinant Biotinylated Cynomolgus CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) is expressed from Expi293 with hFc tag at the C-terminal.; It contains Gln22 - Asp117 (CD3E)&Phe22-Ala105 (CD3D).
Product Category Recombinant Protein
Target CD3E&CD3D/CD3 epsilon&CD3 delta
Synonyms CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon
Gene/Protein ID Q95LI5.2 (CD3E)&Q95LI8 (CD3D)
Species Cynomolgus
Exact Sequence Gln22-Asp117 (CD3E)&Phe22-Ala105 (CD3D)
Molecular Weight The protein has a predicted MW of 36.9 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation, the protein migrates to 58-62 kDa and 44-46 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-CD3, hFc Tag Antibody at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E&CD3D, hFc Tag with the EC50 of 58.2ng/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-CD3, hFc Tag Antibody at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E&CD3D, hFc Tag with the EC50 of 58.2ng/ml determined by ELISA.
The purity of Biotinylated Cynomolgus CD3E&CD3D was greater than 95% as determined by SEC-HPLC.
Biotinylated Cynomolgus CD3E&CD3D on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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