Name: Biotinylated Cynomolgus FAP Protein (FAP-CM401B)
Brand: KactusBio
SKU: FAP-CM401B-100UG
Price: 742.5 USD
Availability: InStock

Biotinylated Cynomolgus FAP Protein (FAP-CM401B)

Immobilized Biotinylated Cynomolgus FAP, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA (QC Test).

Biotinylated Cynomolgus FAP on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

The purity of Biotinylated Cynomolgus FAP is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Cynomolgus FAP, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Leu26-Asp760

Catalog #FAP-CM401B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Cynomolgus FAP Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Leu26-Asp760.
Product Background	Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Protein	FAP
Synonyms	FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA
Accession	XP_005573377
Species	Cynomolgus
Biotinylated	yes
Amino Acid Range	Leu26-Asp760
Molecular Weight	The protein has a predicted MW of 88 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Cynomolgus FAP, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	N-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 20mM Tris, 500mM NaCl, 200mM L-arginine (pH 8.2). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Cynomolgus FAP, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA (QC Test).
	Biotinylated Cynomolgus FAP on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
	The purity of Biotinylated Cynomolgus FAP is greater than 95% as determined by SEC-HPLC.