Biotinylated Cynomolgus FAP Protein (FAP-CM401B)
Biotinylated Cynomolgus FAP Protein (FAP-CM401B)
Biotinylated Cynomolgus FAP Protein (FAP-CM401B)
Biotinylated Cynomolgus FAP Protein (FAP-CM401B)
  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Cynomolgus FAP, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Leu26-Asp760
Catalog #FAP-CM401B
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Product Description
 Recombinant Biotinylated Cynomolgus FAP Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Leu26-Asp760.
Product Background
 Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells.
Product Category
 Recombinant Protein
Target
 FAP
Synonyms
 FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA
Accession
Species
 Cynomolgus
Biotinylated
 yes
Amino Acid Range
 Leu26-Asp760
Molecular Weight
 The protein has a predicted MW of 88 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Cynomolgus FAP, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
 N-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 20mM Tris, 500mM NaCl, 200mM L-arginine (pH 8.2). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Cynomolgus FAP, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA (QC Test).
Biotinylated Cynomolgus FAP on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus FAP is greater than 95% as determined by SEC-HPLC.

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