Biotinylated Cynomolgus FGL2 Protein (FGL-CM612B)
Biotinylated Cynomolgus FGL2 Protein (FGL-CM612B)
  • Express System: HEK293
  • Product Tag: N-His-Avi,N-Flag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Cynomolgus FGL2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FGL2 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Val205-Pro439
Catalog #FGL-CM612B
Regular price $525.00
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Product Description
Recombinant Biotinylated Cynomolgus FGL2 Protein is expressed from HEK293 with His tag and Avi tag and Flag tag at the N-Terminus.It contains Val205-Pro439.
Product Background
Fibrinogen-like protein 2 (FGL2) is a member of the fibrinogen-like protein family and possesses important regulatory functions in both innate and adaptive immune responses. FGL2 is overexpressed in glioma, and its expression level is negatively associated with the prognosis of glioma patients.
Product Category
Recombinant Protein
Target
FGL2
Accession
Species
Cynomolgus
Biotinylated
yes
Amino Acid Range
Val205-Pro439
Molecular Weight
The protein has a predicted MW of 31.40 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Cynomolgus FGL2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FGL2 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA. See testing image for detail.
Product Tag
N-His-Avi,N-Flag
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Cynomolgus FGL2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FGL2 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA.
Biotinylated Cynomolgus FGL2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus FGL2 is greater than 95% as determined by SEC-HPLC.

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