Biotinylated Cynomolgus FGL2 Protein (FGL-CM612B)

  • Express System: HEK293
  • Product Tag: N-His-Avi,N-Flag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Cynomolgus FGL2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FGL2 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Val205-Pro439

Catalog: FGL-CM612B

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Product Description Recombinant Biotinylated Cynomolgus FGL2 Protein is expressed from HEK293 with His tag and Avi tag and Flag tag at the N-Terminus.It contains Val205-Pro439.

Product Background

Fibrinogen-like protein 2 (FGL2) is a member of the fibrinogen-like protein family and possesses important regulatory functions in both innate and adaptive immune responses. FGL2 is overexpressed in glioma, and its expression level is negatively associated with the prognosis of glioma patients.

Product Category

Recombinant Protein

Target

FGL2

Gene/Protein ID

A0A2K5WID3

Species

Cynomolgus

Exact Sequence

Val205-Pro439
Molecular Weight The protein has a predicted MW of 31.40 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Biotinylated Cynomolgus FGL2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FGL2 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA. See testing image for detail.

Product Tag

N-His-Avi,N-Flag

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Cynomolgus FGL2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FGL2 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA.
The purity of Biotinylated Cynomolgus FGL2 is greater than 95% as determined by SEC-HPLC.
Biotinylated Cynomolgus FGL2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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