Name: Biotinylated Cynomolgus FGL2 Protein (FGL-CM612B)
Brand: KactusBio
SKU: FGL-CM612B-100UG
Price: 577.5 USD
Availability: InStock

ELISA binding curve showing the interaction between biotinylated Cynomolgus FGL2 protein and anti-FGL2 antibody, measured by OD450.

Biotinylated Cynomolgus FGL2 Protein (FGL-CM612B)

Immobilized Biotinylated Cynomolgus FGL2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FGL2 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA.

Biotinylated Cynomolgus FGL2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Cynomolgus FGL2 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His-Avi,N-Flag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Cynomolgus FGL2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FGL2 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Val205-Pro439

Catalog #FGL-CM612B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Cynomolgus FGL2 Protein is expressed from HEK293 with His tag and Avi tag and Flag tag at the N-Terminus.It contains Val205-Pro439.
Product Background	Fibrinogen-like protein 2 (FGL2) is a member of the fibrinogen-like protein family and possesses important regulatory functions in both innate and adaptive immune responses. FGL2 is overexpressed in glioma, and its expression level is negatively associated with the prognosis of glioma patients.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	FGL2
Synonyms	Fibroleukin; pT49; FGL2; fibrinogen-like 2;
Accession	A0A2K5WID3
Species	Cynomolgus
Biotinylated	yes
Amino Acid Range	Val205-Pro439
Molecular Weight	The protein has a predicted MW of 31.40 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Cynomolgus FGL2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FGL2 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His-Avi,N-Flag
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Cynomolgus FGL2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FGL2 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA.
	Biotinylated Cynomolgus FGL2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Cynomolgus FGL2 is greater than 95% as determined by SEC-HPLC.