Biotinylated Cynomolgus IL-10 R alpha Protein (Primary Amine Labeling) (IL1-CM1RAB)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-10, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-10 R alpha, His Tag with the EC50 of 49.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: His22-Asn233
Catalog #IL1-CM1RAB
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Product Description
 Recombinant Biotinylated Cynomolgus IL-10 R alpha Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-terminus.It contains His22-Asn233.
Product Background
 The interleukin-10 receptor alpha (IL10RA) gene codes for the alpha chain of the IL-10 receptor which binds the cytokine IL-10. IL-10 is an anti-inflammatory cytokine with immunoregulatory function during the pathogenesis of many inflammatory disorders in livestock, including Johne's disease (JD). JD is a chronic enteritis in cattle caused by Mycobacterium avium subsp. paratuberculosis (MAP) and is responsible for significant economic losses to the dairy industry. Several candidate genes including IL10RA have been found to be associated with JD.
Product Category
 Recombinant Protein
Target
 IL-10 R alpha
Synonyms
 IL-10RA; IL10RA; IL10R; IL-10R1; IL-10R subunit 1; CDw210a; HIL-10R; IBD28; IL-10 R alpha
Accession
Species
 Cynomolgus
Biotinylated
 yes
Amino Acid Range
 His22-Asn233
Molecular Weight
 The protein has a predicted MW of 25.34 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-10, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-10 R alpha, His Tag with the EC50 of 49.8ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-10, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-10 R alpha, His Tag with the EC50 of 49.8ng/ml determined by ELISA.
Biotinylated Cynomolgus IL-10 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus IL-10 R alpha is greater than 95% as determined by SEC-HPLC.

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