• Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-6 R alpha, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-6, His-Avi Tag with the EC50 of 0.16ug/ml determined by ELISA (QC Test).
  • Amino Acid Range: Ala28-Met212
Catalog #IL6-CM401B
Price
Regular price $577.50
Regular price Sale price $577.50
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Cynomolgus IL-6 Protein (IL6-CM401B) Summary

Product Description

 Recombinant Biotinylated Cynomolgus IL-6 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus.It contains Ala28-Met212.

Product Background

 IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression.Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Biotinylated Cynomolgus IL-6 Protein (IL6-CM401B) Specifications/Details

Protein
 IL-6
Synonyms
 IFN-beta-2; IL6; IL-6; BSF-2; CDF; MGI-2A; Interleukin-6; HSF; IFNB2; HGF;IFN-β-2
Accession
Species
 Cynomolgus
Biotinylated
 yes
Amino Acid Range
 Ala28-Met212
Molecular Weight
 The protein has a predicted MW of 23.87 kDa. Due to glycosylation, the protein migrates to 27-35 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-6 R alpha, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-6, His-Avi Tag with the EC50 of 0.16ug/ml determined by ELISA (QC Test).
Product Tag
 N-His-Avi

Fluorophore

 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.； -80°C for 3 months after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human IL-6 R alpha, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-6, His-Avi Tag with the EC50 of 0.16ug/ml determined by ELISA (QC Test). Immobilized Human IL-6 R alpha, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-6, His-Avi Tag with the EC50 of 0.16ug/ml determined by ELISA (QC Test).
Biotinylated Cynomolgus IL-6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Cynomolgus IL-6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus IL-6 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Cynomolgus IL-6 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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