Biotinylated Cynomolgus IL-6 Protein (IL6-CM401B)
  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-6 R alpha, hFc Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-6, His-Avi Tag with the EC50 of 0.16ug/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Ala28-Met212
Catalog #IL6-CM401B
Regular price $577.50
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Biotinylated Cynomolgus IL-6 Protein (IL6-CM401B) Summary

Product Description

Recombinant Biotinylated Cynomolgus IL-6 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus.It contains Ala28-Met212.

Product Background

IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression.Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Biotinylated Cynomolgus IL-6 Protein (IL6-CM401B) Specifications/Details

Protein
IL-6
Synonyms
IFN-beta-2; IL6; IL-6; BSF-2; CDF; MGI-2A; Interleukin-6; HSF; IFNB2; HGF;IFN-β-2
Accession
Species
Cynomolgus
Biotinylated
yes
Amino Acid Range
Ala28-Met212
Molecular Weight
The protein has a predicted MW of 23.87 kDa. Due to glycosylation, the protein migrates to 27-35 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human IL-6 R alpha, hFc Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-6, His-Avi Tag with the EC50 of 0.16ug/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

N-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human IL-6 R alpha, hFc Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-6, His-Avi Tag with the EC50 of 0.16ug/ml determined by ELISA (QC Test). Immobilized Human IL-6 R alpha, hFc Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-6, His-Avi Tag with the EC50 of 0.16ug/ml determined by ELISA (QC Test).
Biotinylated Cynomolgus IL-6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Cynomolgus IL-6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Human IL-6 R alpha, hFc Tag on ProA-Biosensor can bind Biotinylated Cynomolgus IL-6, His-Avi Tag with an affinity constant of 12.40 nM as determined in BLI assay (Gator® Prime). Loaded Human IL-6 R alpha, hFc Tag on ProA-Biosensor can bind Biotinylated Cynomolgus IL-6, His-Avi Tag with an affinity constant of 12.40 nM as determined in BLI assay (Gator® Prime).
The purity of Biotinylated Cynomolgus IL-6 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Cynomolgus IL-6 is greater than 95% as determined by SEC-HPLC.
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