Biotinylated Cynomolgus/Rhesus macaque LRRC15/LIB Protein (LRR-CM415B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-LRRC15 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus/Rhesus macaque LRRC15, His Tag with the EC50 of 0.20ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Tyr22-Gly538

Catalog: LRR-CM415B

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Product Description Recombinant Biotinylated Cynomolgus/Rhesus macaque LRRC15/LIB Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Tyr22-Gly538.

Product Background

The chimeric protein binds in vitro and in vivo to a specific element upstream of LRRC15, leading to dramatic transcriptional activation.- LRRC15 encodes a leucine-rich transmembrane protein, present at the leading edge of migrating cells, the expression of which in normal tissues is restricted to the invasive cytotrophoblast layer of the placenta; small interfering (siRNA)-mediated suppression of LRRC15 expression in breast cancer cells leads to abrogation of invasiveness in vitro.

Product Category

Recombinant Protein

Target

LRRC15/LIB

Gene/Protein ID

A0A2K5UKB6/F7FSB9

Species

Cynomolgus/Rhesus macaque

Exact Sequence

Tyr22-Gly538
Molecular Weight The protein has a predicted MW of 58.92 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Anti-LRRC15 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus/Rhesus macaque LRRC15, His Tag with the EC50 of 0.20ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Anti-LRRC15 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus/Rhesus macaque LRRC15, His Tag with the EC50 of 0.20ug/ml determined by ELISA.
The purity of Biotinylated Cynomolgus/Rhesus macaque LRRC15 is greater than 95% as determined by SEC-HPLC.
Biotinylated Cynomolgus/Rhesus macaque LRRC15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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