Product Description |
Recombinant Biotinylated Cynomolgus/Rhesus macaque LRRC15/LIB Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Tyr22-Gly538. |
Product Background |
The chimeric protein binds in vitro and in vivo to a specific element upstream of LRRC15, leading to dramatic transcriptional activation.- LRRC15 encodes a leucine-rich transmembrane protein, present at the leading edge of migrating cells, the expression of which in normal tissues is restricted to the invasive cytotrophoblast layer of the placenta; small interfering (siRNA)-mediated suppression of LRRC15 expression in breast cancer cells leads to abrogation of invasiveness in vitro. |
Product Category |
Recombinant Protein |
Target |
LRRC15/LIB |
Accession |
|
Species |
Cynomolgus/Rhesus macaque |
Biotinylated |
yes |
Amino Acid Range |
Tyr22-Gly538 |
Molecular Weight |
The protein has a predicted MW of 61.50 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Anti-LRRC15 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus/Rhesus macaque LRRC15, His Tag with the EC50 of 75.9ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Anti-LRRC15 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus/Rhesus macaque LRRC15, His Tag with the EC50 of 75.9ng/ml determined by ELISA. |
|
|
Biotinylated Cynomolgus/Rhesus macaque LRRC15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Cynomolgus/Rhesus macaque LRRC15 is greater than 95% as determined by SEC-HPLC. |
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