Name: Biotinylated Human ALCAM/CD166 Protein (Primary Amine Labeling) (ALC-HM101B)
Brand: KactusBio
SKU: ALC-HM101B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human ALCAM/CD166 Protein (Primary Amine Labeling) (ALC-HM101B)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Anti-ALCAM Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ALCAM, His Tag with the EC50 of 2.62ug/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Trp28-Ala526

Catalog #ALC-HM101B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human ALCAM/CD166 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Trp28-Ala526.
Product Background	Brain metastasis (BM) in non-small-cell lung cancer (NSCLC) has a very poor prognosis. Recent studies have demonstrated the importance of cell adhesion molecules in tumor metastasis.Elevated levels of ALCAM expression promote BM formation in NSCLC through increased tumor cell dissemination and interaction with the brain endothelial cells. Therefore, ALCAM could be targeted to reduce the occurrence of BM.
Product Category	Recombinant Protein

Target	ALCAM/CD166
Synonyms	CD166; ALCAM; MEMD; CD6L; KG-CAM
Accession	Q13740-1
Species	Human
Biotinylated	yes
Amino Acid Range	Trp28-Ala526
Molecular Weight	The protein has a predicted MW of 57 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Anti-ALCAM Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ALCAM, His Tag with the EC50 of 2.62ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Anti-ALCAM Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ALCAM, His Tag with the EC50 of 2.62ug/ml determined by ELISA.
	Biotinylated Human ALCAM (Primary Amine Labeling) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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