Biotinylated Human ANGPTL3/Angiopoietin-like 3 Protein (ANG-HM403B)
Biotinylated Human ANGPTL3/Angiopoietin-like 3 Protein (ANG-HM403B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Anti-ANGPTL3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ANGPTL3, His Tag with the EC50 of 10.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ser17-Lys219
Catalog #ANG-HM403B
Regular price $525.00
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Product Description

Recombinant Biotinylated Human ANGPTL3/Angiopoietin-like 3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser17-Lys219.

Product Background

ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins. Mature human ANGPTL3 contains an N-terminal coiled coil domain and a C‑terminal fibrinogen-like domain. ANGPTL3 is expressed in the liver from early in development through adulthood. Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake.

Product Category

Recombinant Protein / Antibody discovery

Protein

ANGPTL3/Angiopoietin-like 3

Synonyms

ANGPTL3; ANGPT5; ANG-5; Angiopoietin-5; FHBL2; ANL3; Angiopoietin-5; Angiopoietin-like Protein 3

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Ser17-Lys219

Molecular Weight

The protein has a predicted MW of 26.6 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Anti-ANGPTL3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ANGPTL3, His Tag with the EC50 of 10.2ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Anti-ANGPTL3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ANGPTL3, His Tag with the EC50 of 10.2ng/ml determined by ELISA. Immobilized Anti-ANGPTL3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ANGPTL3, His Tag with the EC50 of 10.2ng/ml determined by ELISA.
Biotinylated Human ANGPTL3 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Biotinylated Human ANGPTL3 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Biotinylated Human ANGPTL3 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human ANGPTL3 is greater than 95% as determined by SEC-HPLC.

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