Biotinylated Human ANGPTL3/Angiopoietin-like 3 Protein (ANG-HM403B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-ANGPTL3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ANGPTL3, His Tag with the EC50 of 5.0ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ser17-Lys219

Catalog: ANG-HM403B

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Product Description Recombinant Biotinylated Human ANGPTL3/Angiopoietin-like 3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser17-Lys219.

Product Background

ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins. Mature human ANGPTL3 contains an N-terminal coiled coil domain and a C‑terminal fibrinogen-like domain. ANGPTL3 is expressed in the liver from early in development through adulthood. Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake.

Product Category

Recombinant Protein

Target

ANGPTL3/Angiopoietin-like 3
Synonyms ANGPTL3; ANGPT5; ANG-5; Angiopoietin-5; FHBL2; ANL3; Angiopoietin-5; Angiopoietin-like Protein 3

Gene/Protein ID

Q9Y5C1

Species

Human

Exact Sequence

Ser17-Lys219
Molecular Weight The protein has a predicted MW of 26.6 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Anti-ANGPTL3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ANGPTL3, His Tag with the EC50 of 5.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-ANGPTL3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ANGPTL3, His Tag with the EC50 of 5.0ng/ml determined by ELISA.
The purity of Biotinylated Human ANGPTL3 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human ANGPTL3 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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