Biotinylated Human AXL Protein (AXL-HM401B)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Biotinylated: yes
Activity: Immobilized Biotinylated Biotinylated Human Axl, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-AXL Antibody, hFc Tag with the EC50 of 10.2ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ala26-Pro449

Catalog: AXL-HM401B

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Product Description	Recombinant Biotinylated Human AXL Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala26-Pro449.
Product Background	Axl, a member of the TAM (Tyro3, Axl, Mer) family, and its inhibitors can specifically break the kinase signaling nodes, allowing advanced patients to regain drug sensitivity with improved therapeutic efficacy. Overexpression and activation of Axl receptor tyrosine kinase have been widely accepted to promote cell proliferation, chemotherapy resistance, invasion, and metastasis in several human cancers, such as lung, breast, and pancreatic cancers.
Product Category	Recombinant Protein

Target	AXL
Synonyms	Axl; UFO; AXL oncogene; AXL; ARK; JTK11; Tyro7; AI323647; EC 2.7.10; EC 2.7.10.1
Gene/Protein ID	P30530-1
Species	Human
Exact Sequence	Ala26-Pro449
Molecular Weight	The protein has a predicted MW of 48.7 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Biotinylated Biotinylated Human Axl, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-AXL Antibody, hFc Tag with the EC50 of 10.2ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Biotinylated Biotinylated Human Axl, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-AXL Antibody, hFc Tag with the EC50 of 10.2ng/ml determined by ELISA.
	The purity of Biotinylated Human AXL is greater than 95% as determined by SEC-HPLC.
	Biotinylated Human AXL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.