HPLC chromatogram with a prominent peak at 7.705 minutes, indicating high purity of the analyzed protein sample.
Biotinylated Human AXL Protein (AXL-HM401B)
Biotinylated Human AXL Protein (AXL-HM401B)
Biotinylated Human AXL Protein (AXL-HM401B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Biotinylated Human Axl, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-AXL Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala26-Pro449
Catalog #AXL-HM401B
Regular price $577.50
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Product Description

Recombinant Biotinylated Human AXL Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala26-Pro449.

Product Background

Axl, a member of the TAM (Tyro3, Axl, Mer) family, and its inhibitors can specifically break the kinase signaling nodes, allowing advanced patients to regain drug sensitivity with improved therapeutic efficacy. Overexpression and activation of Axl receptor tyrosine kinase have been widely accepted to promote cell proliferation, chemotherapy resistance, invasion, and metastasis in several human cancers, such as lung, breast, and pancreatic cancers.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others
Protein
AXL
Synonyms
Axl; UFO; AXL oncogene; AXL; ARK; JTK11; Tyro7; AI323647; EC 2.7.10; EC 2.7.10.1
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Ala26-Pro449
Molecular Weight
The protein has a predicted MW of 48.7 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Biotinylated Human Axl, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-AXL Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Biotinylated Human Axl, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-AXL Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA. Immobilized Biotinylated Biotinylated Human Axl, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-AXL Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA.
Biotinylated Human AXL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human AXL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human AXL is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human AXL is greater than 95% as determined by SEC-HPLC.
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