Name: Biotinylated Human Axl Protein (Primary Amine Labeling) (AXL-HM201B)
Brand: KactusBio
SKU: AXL-HM201B-100UG
Price: 577.5 USD
Availability: InStock

ELISA standard curve showing binding of anti-Axl antibody to biotinylated human Axl, with OD450 absorbance values increasing with concentration.

Biotinylated Human Axl Protein (Primary Amine Labeling) (AXL-HM201B)

Immobilized Human GAS6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Axl, hFc Tag with the EC50 of 9.2ng/ml determined by ELISA (QC Test).

Immobilized Anti-Axl Antibody at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Axl, hFc Tag with the EC50 of 26.8ng/ml determined by ELISA.

Biotinylated Human Axl on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human Axl is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human GAS6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Axl, hFc Tag with the EC50 of 9.2ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Ala26-Pro449

Catalog #AXL-HM201B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human Axl Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala26-Pro449.
Product Background	Axl, a member of the TAM (Tyro3, Axl, Mer) family, and its inhibitors can specifically break the kinase signaling nodes, allowing advanced patients to regain drug sensitivity with improved therapeutic efficacy. Overexpression and activation of Axl receptor tyrosine kinase have been widely accepted to promote cell proliferation, chemotherapy resistance, invasion, and metastasis in several human cancers, such as lung, breast, and pancreatic cancers.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	AXL
Synonyms	Axl; UFO; AXL oncogene; AXL; ARK; JTK11; Tyro7; AI323647; EC 2.7.10; EC 2.7.10.1
Accession	P30530-1
Species	Human
Biotinylated	yes
Amino Acid Range	Ala26-Pro449
Molecular Weight	The protein has a predicted MW of 72.6 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human GAS6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Axl, hFc Tag with the EC50 of 9.2ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human GAS6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Axl, hFc Tag with the EC50 of 9.2ng/ml determined by ELISA (QC Test).
	Immobilized Anti-Axl Antibody at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Axl, hFc Tag with the EC50 of 26.8ng/ml determined by ELISA.
	Biotinylated Human Axl on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human Axl is greater than 95% as determined by SEC-HPLC.