Biotinylated Human B7-H3 (4Ig) /B7-H3b Protein (BH7-HM43BB)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-B7-H3 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human B7-H3 (4Ig) , His Tag with the EC50 of 8.8ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gly27-Thr461

Catalog: BH7-HM43BB

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Product Description Recombinant Biotinylated Human B7-H3 (4Ig)/B7-H3b Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly27-Thr461.

Product Background

B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.

Product Category

Recombinant Protein

Target

B7-H3 (4Ig)/B7-H3b
Synonyms B7-H3; CD276; PSEC0249;-4Ig-B7-H3; UNQ309; PRO352; B7 homolog 3; CD276
Accession

Species

Human

Exact Sequence

Gly27-Thr461
Molecular Weight The protein has a predicted MW of 49.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Anti-B7-H3 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human B7-H3 (4Ig) , His Tag with the EC50 of 8.8ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-B7-H3 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human B7-H3 (4Ig) , His Tag with the EC50 of 8.8ng/ml determined by ELISA.
The purity of Biotinylated Human B7-H3 (4Ig) is greater than 95% as determined by SEC-HPLC.
Biotinylated Human B7-H3 (4Ig) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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