Biotinylated Human B7-H3/CD276 Protein (BH7-HM473B)
Biotinylated Human B7-H3/CD276 Protein (BH7-HM473B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human B7-H3, His Tag at 0.5 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 2.8 ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Leu29-Pro245
Catalog #BH7-HM473B
Regular price $525.00
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Product Description

Recombinant Biotinylated Human B7-H3/CD276 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu29-Pro245.

Product Background

B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers. B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.

Product Category

Recombinant Protein / Antibody discovery / CAR-T targets

Protein

B7-H3/CD276

Synonyms

B7H3; B7-H3; CD276; PSEC0249;-UNQ309; PRO352; B7 homolog 3; CD276

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Leu29-Pro245

Molecular Weight

The protein has a predicted MW of 26.49 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated Human B7-H3, His Tag at 0.5 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 2.8 ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 24 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human B7-H3, His Tag at 0.5 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 2.8 ng/ml determined by ELISA. Immobilized Biotinylated Human B7-H3, His Tag at 0.5 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 2.8 ng/ml determined by ELISA.
Biotinylated Human B7-H3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human B7-H3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human B7-H3 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human B7-H3 is greater than 95% as determined by SEC-HPLC.

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