Product Description |
Recombinant Biotinylated Human B7-H3/CD276 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu29-Pro245. |
Product Background |
B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction. |
Product Category |
Recombinant Protein |
Target |
B7-H3/CD276 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Leu29-Pro245 |
Molecular Weight |
The protein has a predicted MW of 26.49 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human B7-H3, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Biotinylated Human B7-H3, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA. |
|
|
Biotinylated Human B7-H3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Biotinylated Human B7-H3 is greater than 95% as determined by SEC-HPLC. |
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