Biotinylated Human B7-H3/CD276 Protein (BH7-HM473B)
Biotinylated Human B7-H3/CD276 Protein (BH7-HM473B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human B7-H3, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Leu29-Pro245
Catalog #BH7-HM473B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human B7-H3/CD276 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu29-Pro245.
Product Background
B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.
Product Category
Recombinant Protein
Target
B7-H3/CD276
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Leu29-Pro245
Molecular Weight
The protein has a predicted MW of 26.49 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human B7-H3, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human B7-H3, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA.
Biotinylated Human B7-H3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human B7-H3 is greater than 95% as determined by SEC-HPLC.

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