Name: Biotinylated Human BTLA Protein (BTL-HM401B)
Brand: KactusBio
SKU: BTL-HM401B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human BTLA Protein (BTL-HM401B)

Immobilized Anti-BTLA Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BTLA, His Tag with the EC50 of 8.0ng/ml determined by ELISA.

Immobilized Biotinylated Human BTLA, His Tag at 2ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human HVEM, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA (QC Test).

Biotinylated Human BTLA-on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human BTLA is greater than 95% as determined by SEC-HPLC

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Anti-BTLA Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BTLA, His Tag with the EC50 of 8.0ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Lys31-Ser150

Catalog #BTL-HM401B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human BTLA Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys31-Ser150.
Product Background	B- and T-lymphocyte attenuator (BTLA; CD272) is a 35 kDa type I transmembrane glycoprotein in the CD28 family of T cell costimulatory molecules. BTLA is a inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2. BTLA may interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	BTLA
Synonyms	CD272; BTLA; BTLA1; FLJ16065; MGC129743
Accession	Q7Z6A9-1
Species	Human
Biotinylated	yes
Amino Acid Range	Lys31-Ser150
Molecular Weight	The protein has a predicted MW of 16.7 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Anti-BTLA Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BTLA, His Tag with the EC50 of 8.0ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Anti-BTLA Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BTLA, His Tag with the EC50 of 8.0ng/ml determined by ELISA.
	Immobilized Biotinylated Human BTLA, His Tag at 2ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human HVEM, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA (QC Test).
	Biotinylated Human BTLA-on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human BTLA is greater than 95% as determined by SEC-HPLC