Biotinylated Human BTLA Protein (BTL-HM401B)
Catalog: BTL-HM401B
Order now get it on
Product Description | Recombinant Biotinylated Human BTLA Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys31-Ser150. |
Product Background |
B- and T-lymphocyte attenuator (BTLA; CD272) is a 35 kDa type I transmembrane glycoprotein in the CD28 family of T cell costimulatory molecules. BTLA is a inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2. BTLA may interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14. |
Product Category |
Recombinant Protein |
Target |
BTLA |
Synonyms | CD272; BTLA; BTLA1; FLJ16065; MGC129743 |
Accession | |
Species |
Human |
Amino Acid Range |
Lys31-Ser150 |
Molecular Weight | The protein has a predicted MW of 16.7 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Anti-BTLA Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BTLA, His Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
You may also be interested in
View more associated products