Biotinylated Human BTLA Protein (BTL-HM401B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Anti-BTLA Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BTLA, His Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Lys31-Ser150
Catalog #BTL-HM401B
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Product Description
 Recombinant Biotinylated Human BTLA Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys31-Ser150.
Product Background
 B- and T-lymphocyte attenuator (BTLA; CD272) is a 35 kDa type I transmembrane glycoprotein in the CD28 family of T cell costimulatory molecules. BTLA is a inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2. BTLA may interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14.
Product Category
 Recombinant Protein
Target
 BTLA
Synonyms
 CD272; BTLA; BTLA1; FLJ16065; MGC129743
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Lys31-Ser150
Molecular Weight
 The protein has a predicted MW of 16.7 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Anti-BTLA Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BTLA, His Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-BTLA Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BTLA, His Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test).
Immobilized Human HVEM, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human BTLA, His Tag with the EC50 of 1.4ug/ml determined by ELISA.
Biotinylated Human BTLA-on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human BTLA is greater than 95% as determined by SEC-HPLC

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