Name: Biotinylated Human BTLA Protein (BTL-HM401B)
Brand: KactusBio
SKU: BTL-HM401B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human BTLA Protein (BTL-HM401B)

Immobilized Biotinylated Human BTLA, His Tag at 2ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human HVEM, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA (QC Test).

Immobilized Anti-BTLA Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BTLA, His Tag with the EC50 of 8.0ng/ml determined by ELISA.

Biotinylated Human BTLA-on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human BTLA is greater than 95% as determined by SEC-HPLC

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Biotinylated Human BTLA, His Tag at 2ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human HVEM, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Lys31-Ser150

Catalog #BTL-HM401B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human BTLA Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys31-Ser150.
Product Background	B- and T-lymphocyte attenuator (BTLA; CD272) is a 35 kDa type I transmembrane glycoprotein in the CD28 family of T cell costimulatory molecules. BTLA is a inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2. BTLA may interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	BTLA
Synonyms	CD272; BTLA; BTLA1; FLJ16065; MGC129743
Accession	Q7Z6A9-1
Species	Human
Biotinylated	yes
Amino Acid Range	Lys31-Ser150
Molecular Weight	The protein has a predicted MW of 16.7 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human BTLA, His Tag at 2ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human HVEM, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human BTLA, His Tag at 2ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human HVEM, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA (QC Test).
	Immobilized Anti-BTLA Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human BTLA, His Tag with the EC50 of 8.0ng/ml determined by ELISA.
	Biotinylated Human BTLA-on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human BTLA is greater than 95% as determined by SEC-HPLC