Product Description |
Recombinant Biotinylated Human CA125/MUC16 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp12783-Ser13467. |
Product Background |
MUC16, also known as the CA125 antigen, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy.Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.Binding to MSLN mediates heterotypic cell adhesion. This may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to MSLN. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets |
Protein |
CA125/MUC16 |
Synonyms |
CA125; CA-125; CA125MUC-16; FLJ14303; MUC16 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Asp12783-Ser13467 |
Molecular Weight |
The protein has a predicted MW of 77.98 kDa. Due to glycosylation, the protein migrates to 90-140 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human MSLN, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC50 of 19.5ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Immobilized Human MSLN, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC50 of 19.5ng/ml determined by ELISA (QC Test). |
|
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Biotinylated Human CA125 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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