Biotinylated Human CD117 Protein (CD1-HM417B)
Biotinylated Human CD117 Protein (CD1-HM417B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human SCF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD117, His Tag with the EC50 of 6.8ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln26-Pro520
Catalog #CD1-HM417B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human CD117 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln26-Pro520.
Product Background
The c-kit proto-oncogen (CD 117) has been shown to be present in several cell types including normal and neoplastic hemopoietic cells. Among normal BM cells, CD117 expression has been found in about half of the CD34 precursors including progenitors committed to the erythroid, granulo-monocytic, and megakaryocytic cell lineages.
Product Category
Recombinant Protein
Target
CD177
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Gln26-Pro520
Molecular Weight
The protein has a predicted MW of 58.60 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human SCF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD117, His Tag with the EC50 of 6.8ug/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human SCF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD117, His Tag with the EC50 of 6.8ug/ml determined by ELISA.
Biotinylated Human CD117 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CD117 is greater than 95% as determined by SEC-HPLC.

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