Name: Biotinylated Human CD117 Protein (CD1-HM417B)
Brand: KactusBio
SKU: CD1-HM417B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human CD117 Protein (CD1-HM417B)

Immobilized Biotinylated Human CD117, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD117 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test).

Immobilized Human SCF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD117, His Tag with the EC50 of 6.8ug/ml determined by ELISA.

Biotinylated Human CD117 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human CD117 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human CD117, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD117 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test).
Amino Acid Range: Gln26-Pro520

Catalog #CD1-HM417B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human CD117 Protein (CD1-HM417B) Summary

Product Description	Recombinant Biotinylated Human CD117 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln26-Pro520.
Product Background	The stem cell factor receptor (SCFR, CD117) is encoded by the c-kit proto-oncogene, which is the cellular counterpart of the v-kit oncogene of a transforming feline retrovirus (HZ4-feline sarcoma virus). It is a 145kD transmembrane glycoprotein which is homologous to several other growth factor receptors such as the receptors for macrophage colony stimulating factor (M-CSF, CSF-1, c-fms), platelet-derived growth factor a and b (PDGF-Ra , PDGF-Rb ), and the newly described growth factor receptor STK-1, which is the human homologue of the murine FLK2/FLT3 receptor.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human CD117 Protein (CD1-HM417B) Specifications/Details

Protein	CD177
Synonyms	SCFR; PBT; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT
Accession	P10721-2
Species	Human
Biotinylated	yes
Amino Acid Range	Gln26-Pro520
Molecular Weight	The protein has a predicted MW of 58.60 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human CD117, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD117 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test).
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human CD117, His-Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CD117 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test).
	Immobilized Human SCF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD117, His Tag with the EC50 of 6.8ug/ml determined by ELISA.
	Biotinylated Human CD117 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human CD117 is greater than 95% as determined by SEC-HPLC.