ELISA binding curve showing the interaction between biotinylated human CD155, hFc tag, and human TIGIT, His tag. The graph plots mean absorbance at 450 nm (OD450) against the logarithmic concentration of biotinylated human CD155, displaying a sigmoidal dose-response relationship
Biotinylated Human CD155/PVR Protein (CD1-HM555B)
  • Express System: HEK293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Biotinylated Human CD155, hFc Tag at 5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human TIGIT, His Tag with the EC50 of 1.14ug/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Trp21-Asn343
Catalog #CD1-HM555B
Regular price $577.50
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Biotinylated Human CD155/PVR Protein (CD1-HM555B) Summary

Product Description

Recombinant Biotinylated Human CD155/PVR Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Trp21-Asn343.

Product Background

CD155 is a cell surface adhesion molecule functioning in tumor cell migration, invasion, and metastasis, and not surprisingly, is also designated as a common tumor-associated antigen. CD155 is also recognized by NK cells to induce their cytotoxicity. CD155 is also commonly referred to as the "poliovirus receptor," or PVR.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Biotinylated Human CD155/PVR Protein (CD1-HM555B) Specifications/Details

Protein
CD155/PVR
Synonyms
CD155; HVED; NECL5; Necl-5; nectin-like 5; PVR; PVS; Tage4; PVSFLJ25946; FLJ25946
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Trp21-Asn343
Molecular Weight
The protein has a predicted MW of 62.82 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human CD155, hFc Tag at 5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human TIGIT, His Tag with the EC50 of 1.14ug/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-hFc-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human CD155, hFc Tag at 5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human TIGIT, His Tag with the EC50 of 1.14ug/ml determined by ELISA (QC Test). Immobilized Biotinylated Human CD155, hFc Tag at 5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human TIGIT, His Tag with the EC50 of 1.14ug/ml determined by ELISA (QC Test).
Biotinylated Human CD155 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human CD155 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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