
- Express System: HEK293
- Product Tag: C-His-Avi
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: The affinity constant of 0.72 uM as determined in SPR assay (Biacore T200).
- Amino Acid Range: Gly25-Leu158
Product Description |
Recombinant Biotinylated Human CD160 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gly25-Leu158. |
Product Background |
CD160 (also Natural killer cell receptor BY55) is a 27 ‑ 30 kDa member of the Ig superfamily. In human, it is expressed principally on nonmyeloid hematopoietic cells. CD160 antigenis a receptor on immune cells capable to deliver stimulatory or inhibitory signals that regulate cell activation and differentiation. Exists as a GPI-anchored and as a transmembrane form, each likely initiating distinct signaling pathways via phosphoinositol 3-kinase in activated NK cells and via LCK and CD247/CD3 zeta chain in activated T cells. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Protein |
CD160 |
Synonyms |
CD160;-BY55; BY55FLJ46513; NK1; NK28 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gly25-Leu158 |
Molecular Weight |
The protein has a predicted MW of 17.7 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.72 uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.; -80°C for 3 months after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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