Name: Biotinylated Human CD160 Protein (CD1-HM460B)
Brand: KactusBio
SKU: CD1-HM460B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human CD160 Protein (CD1-HM460B)

Human HVEM, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human CD160, His Tag with an affinity constant of 0.72 uM as determined in SPR assay (Biacore T200) (QC Test).

Biotinylated Human CD160 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human CD160 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.72 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gly25-Leu158

Catalog #CD1-HM460B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human CD160 Protein (CD1-HM460B) Summary

Product Description	Recombinant Biotinylated Human CD160 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gly25-Leu158.
Product Background	CD160 (also Natural killer cell receptor BY55) is a 27 ‑ 30 kDa member of the Ig superfamily. In human, it is expressed principally on nonmyeloid hematopoietic cells. CD160 antigenis a receptor on immune cells capable to deliver stimulatory or inhibitory signals that regulate cell activation and differentiation. Exists as a GPI-anchored and as a transmembrane form, each likely initiating distinct signaling pathways via phosphoinositol 3-kinase in activated NK cells and via LCK and CD247/CD3 zeta chain in activated T cells.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Biotinylated Human CD160 Protein (CD1-HM460B) Specifications/Details

Protein	CD160
Synonyms	CD160;-BY55; BY55FLJ46513; NK1; NK28
Accession	O95971-1
Species	Human
Biotinylated	yes
Amino Acid Range	Gly25-Leu158
Molecular Weight	The protein has a predicted MW of 17.7 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 0.72 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.； -80°C for 3 months after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human HVEM, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human CD160, His Tag with an affinity constant of 0.72 uM as determined in SPR assay (Biacore T200) (QC Test).
	Biotinylated Human CD160 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human CD160 is greater than 95% as determined by SEC-HPLC.