Biotinylated Human CD2/SRBC Protein (CD2-HM402B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human CD2, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD58, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Lys25-Asp209
Catalog #CD2-HM402B
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Product Description
 Recombinant Biotinylated Human CD2/SRBC Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Lys25-Asp209.
Product Background
 The CD2 family of receptors is evolutionarily conserved and widely expressed on cells within the hematopoietic compartment. In recent years several new members have been identified with important roles in the immune system. CD2 family members regulate natural killer (NK) cell lytic activity and inflammatory cytokine production when engaged by ligands on tumor cells.
Product Category
 Recombinant Protein
Target
 CD2/SRBC
Synonyms
 T11; CD2; SRBC; LFA-2; LFA-3 receptor
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Lys25-Asp209
Molecular Weight
 The protein has a predicted MW of 24.15 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human CD2, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD58, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human CD2, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD58, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA (QC Test).
Biotinylated Human CD2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CD2 is greater than 95% as determined by SEC-HPLC.

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