- Express System: HEK293
- Product Tag: C-His-Avi
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Biotinylated Human CD2, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD58, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA (QC Test). See testing image for detail.
- Amino Acid Range: Lys25-Asp209
Product Description |
Recombinant Biotinylated Human CD2/SRBC Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Lys25-Asp209. |
Product Background |
The CD2 family of receptors is evolutionarily conserved and widely expressed on cells within the hematopoietic compartment. In recent years several new members have been identified with important roles in the immune system. CD2 family members regulate natural killer (NK) cell lytic activity and inflammatory cytokine production when engaged by ligands on tumor cells. |
Product Category |
Recombinant Protein |
Target |
CD2/SRBC |
Synonyms |
T11; CD2; SRBC; LFA-2; LFA-3 receptor |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Lys25-Asp209 |
Molecular Weight |
The protein has a predicted MW of 24.15 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human CD2, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD58, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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