Name: Biotinylated Human CD27/TNFRSF7 Protein (CD2-HM227B)
Brand: KactusBio
SKU: CD2-HM227B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human CD27/TNFRSF7 Protein (CD2-HM227B)

Immobilized Human CD27 Ligand (Trimer), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD27, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test).

Biotinylated Human CD27 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human CD27 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD27 Ligand (Trimer), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD27, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Thr21-Ile192

Catalog #CD2-HM227B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human CD27/TNFRSF7 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-terminus.It contains Thr21-Ile192.
Product Background	CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	CD27/TNFRSF7
Synonyms	CD27 antigen; Tp55; TNFRSF7; S152; T14; S152. LPFS2; MGC20393; S152CD27L receptor
Accession	P26842
Species	Human
Biotinylated	yes
Amino Acid Range	Thr21-Ile192
Molecular Weight	The protein has a predicted MW of 47.01 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CD27 Ligand (Trimer), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD27, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-hFc-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human CD27 Ligand (Trimer), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD27, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test).
	Biotinylated Human CD27 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human CD27 is greater than 95% as determined by SEC-HPLC.