Biotinylated Human CD27/TNFRSF7 Protein (CD2-HM227B)
Biotinylated Human CD27/TNFRSF7 Protein (CD2-HM227B)
  • Express System: HEK293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD27 Ligand (Trimer), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD27, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Thr21-Ile192
Catalog #CD2-HM227B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human CD27/TNFRSF7 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-terminus.It contains Thr21-Ile192.
Product Background
CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis.
Product Category
Recombinant Protein
Target
CD27/TNFRSF7
Synonyms
CD27 antigen; Tp55; TNFRSF7; S152; T14; S152. LPFS2; MGC20393; S152CD27L receptor
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Thr21-Ile192
Molecular Weight
The protein has a predicted MW of 47.01 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human CD27 Ligand (Trimer), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD27, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
C-hFc-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD27 Ligand (Trimer), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD27, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test).
Biotinylated Human CD27 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CD27 is greater than 95% as determined by SEC-HPLC.

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