Biotinylated Human CD27/TNFRSF7 Protein (CD2-HM227B)
  • Express System: HEK293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD27 Ligand (Trimer), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD27, hFc Tag with the EC50 of 81.4ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Thr21-Ile192
Catalog #CD2-HM227B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human CD27/TNFRSF7 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-terminus.It contains Thr21-Ile192.
Product Background
CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis.
Product Category
Recombinant Protein
Target
CD27/TNFRSF7
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Thr21-Ile192
Molecular Weight
The protein has a predicted MW of 47.01 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human CD27 Ligand (Trimer), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD27, hFc Tag with the EC50 of 81.4ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-hFc-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human CD27 Ligand (Trimer), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD27, hFc Tag with the EC50 of 81.4ng/ml determined by ELISA.
Biotinylated Human CD27 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CD27 is greater than 95% as determined by SEC-HPLC.
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