Name: Biotinylated Human CD27/TNFRSF7 Protein (CD2-HM227B)
Brand: KactusBio
SKU: CD2-HM227B-100UG
Price: 577.5 USD
Availability: InStock

ELISA binding curve of biotinylated human CD27 interacting with CD27 ligand trimer, showing dose-dependent increase in OD450 absorbance.

Biotinylated Human CD27/TNFRSF7 Protein (CD2-HM227B)

Immobilized Human CD27 Ligand (Trimer), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD27, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test).

Biotinylated Human CD27 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human CD27 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD27 Ligand (Trimer), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD27, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Thr21-Ile192

Catalog #CD2-HM227B

Size

100UG

500UG

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Biotinylated Human CD27/TNFRSF7 Protein (CD2-HM227B) Summary

Product Description	Recombinant Biotinylated Human CD27/TNFRSF7 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-terminus.It contains Thr21-Ile192.
Product Background	CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Biotinylated Human CD27/TNFRSF7 Protein (CD2-HM227B) Specifications/Details

Protein	CD27/TNFRSF7
Synonyms	CD27 antigen; Tp55; TNFRSF7; S152; T14; S152. LPFS2; MGC20393; S152CD27L receptor
Accession	P26842
Species	Human
Biotinylated	yes
Amino Acid Range	Thr21-Ile192
Molecular Weight	The protein has a predicted MW of 47.01 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CD27 Ligand (Trimer), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD27, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-hFc-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human CD27 Ligand (Trimer), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD27, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test).
	Biotinylated Human CD27 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human CD27 is greater than 95% as determined by SEC-HPLC.