Product Description |
Recombinant Biotinylated Human CD30/TNFRSF8 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe19-Lys379. |
Product Background |
The transmembrane receptor CD30 (TNFRSF8) and its ligand CD30L (CD153, TNFSF8) are members of the tumor necrosis factor (TNF) superfamily and display restricted expression in subpopulations of activated T-and B-cells in nonpathologic conditions. CD30 expression is upregulated in various hematological malignancies, including Reed-Sternberg cells in Hodgkin's disease (HD), anaplastic large cell lymphoma (ALCL) and subsets of Non-Hodgkin's lymphomas (NHLs). |
Product Category |
Recombinant Protein |
Target |
CD30/TNFRSF8 |
Synonyms |
CD30; CD30KI-1; CD30L receptor; TNFRSF8; D1S166EKi-1; CD30KI-1 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Phe19-Lys379 |
Molecular Weight |
The protein has a predicted MW of 41.3 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Anti-CD30 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD30, His Tag with the EC50 of 11.5ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Anti-CD30 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD30, His Tag with the EC50 of 11.5ng/ml determined by ELISA. |
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Immobilized Human CD30 Ligand, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD30, His Tag with the EC50 of 17.5ng/ml determined by ELISA (QC Test). |
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Biotinylated CD30 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated CD30 is greater than 95% as determined by SEC-HPLC. |
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