Name: Biotinylated Human CD30/TNFRSF8 Protein (CD3-HM430B)
Brand: KactusBio
SKU: CD3-HM430B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human CD30/TNFRSF8 Protein (CD3-HM430B)

Immobilized Human CD30 Ligand, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD30, His Tag with the EC50 of 17.5ng/ml determined by ELISA (QC Test).

Immobilized Anti-CD30 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD30, His Tag with the EC50 of 11.5ng/ml determined by ELISA.

Biotinylated CD30 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated CD30 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD30 Ligand, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD30, His Tag with the EC50 of 17.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Phe19-Lys379

Catalog #CD3-HM430B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human CD30/TNFRSF8 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe19-Lys379.
Product Background	The transmembrane receptor CD30 (TNFRSF8) and its ligand CD30L (CD153, TNFSF8) are members of the tumor necrosis factor (TNF) superfamily and display restricted expression in subpopulations of activated T-and B-cells in nonpathologic conditions. CD30 expression is upregulated in various hematological malignancies, including Reed-Sternberg cells in Hodgkin's disease (HD), anaplastic large cell lymphoma (ALCL) and subsets of Non-Hodgkin's lymphomas (NHLs).
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Protein	CD30/TNFRSF8
Synonyms	CD30; CD30KI-1; CD30L receptor; TNFRSF8; D1S166EKi-1; CD30KI-1
Accession	P28908-1
Species	Human
Biotinylated	yes
Amino Acid Range	Phe19-Lys379
Molecular Weight	The protein has a predicted MW of 41.3 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CD30 Ligand, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD30, His Tag with the EC50 of 17.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human CD30 Ligand, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD30, His Tag with the EC50 of 17.5ng/ml determined by ELISA (QC Test).
	Immobilized Anti-CD30 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD30, His Tag with the EC50 of 11.5ng/ml determined by ELISA.
	Biotinylated CD30 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated CD30 is greater than 95% as determined by SEC-HPLC.