ELISA binding curve of biotinylated human CD38 His tag protein detected using anti-CD38 antibody, showing mean absorbance (OD450) vs. antibody concentration.
Biotinylated Human CD38 Protein (CD3-HM138B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human CD38, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Anti-CD38 Antibody, hFc Tag with the EC50 of 8.1ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Val43-Ile300
Catalog #CD3-HM138B
Regular price $577.50
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Biotinylated Human CD38 Protein (CD3-HM138B) Summary

Product Description

Recombinant Biotinylated Human CD38 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val43-Ile300.

Product Background

CD38 (cluster of differentiation 38), also known as cyclic ADP ribose hydrolase, is a transmembrane glycoprotein found on the surface of some immune cells including plasma cells, activated or immature T and B cells, monocytes, and natural killer cells. CD38 participates in cell adhesion, signal transduction and calcium signaling.

Product Category

Recombinant Protein / Antibody discovery / CAR-T targets

Biotinylated Human CD38 Protein (CD3-HM138B) Specifications/Details

Protein
CD38
Synonyms
ADPRC 1; T10; CD38; ADP-ribosyl cyclase 1; cADPr hydrolase 1; CD38 molecule; EC 2.4.99.20; EC 3.2.2.6
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Val43-Ile300
Molecular Weight
The protein has a predicted MW of 32.50 kDa. Due to glycosylation, the protein migrates to 42-50 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human CD38, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Anti-CD38 Antibody, hFc Tag with the EC50 of 8.1ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human CD38, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Anti-CD38 Antibody, hFc Tag with the EC50 of 8.1ng/ml determined by ELISA. Immobilized Biotinylated Human CD38, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Anti-CD38 Antibody, hFc Tag with the EC50 of 8.1ng/ml determined by ELISA.
Biotinylated Human CD38 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human CD38 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CD38 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human CD38 is greater than 95% as determined by SEC-HPLC.
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