Product Description |
Recombinant Biotinylated Human CD3E/CD3 epsilon 1-27 Protein is expressed from HEK293 with hFc tag and Avi Tag at the C-Terminus.It contains Asp23-Thr48. |
Product Background |
CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains. |
Product Category |
Recombinant Protein |
Target |
CD3E/CD3 epsilon 1-27 |
Synonyms |
CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Asp23-Thr48 |
Molecular Weight |
The protein has a predicted MW of 31.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Anti-CD3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD3E 1-27, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Anti-CD3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD3E 1-27, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. |
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Biotinylated Human CD3E 1-27 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human CD3E 1-27 is greater than 95% as determined by SEC-HPLC. |
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