Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-HM505B)
Catalog: CD3-HM505B
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| Product Description | Recombinant Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from Expi293 with hFc tag and Avi tag at the C-terminal.; It contains Asp23-Asp126 (CD3E) & Phe22-Ala105 (CD3D). |
| Product Category | Recombinant Protein |
| Target | CD3E&CD3D/CD3 epsilon&CD3 delta |
| Synonyms | CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon |
| Gene/Protein ID | P07766 (CD3E)&P04234 (CD3D) |
| Species | Human |
| Exact Sequence | Asp23-Asp126 (CD3E)&Phe22-Ala105 (CD3D) |
| Molecular Weight | The protein has a predicted MW of 40 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Biotinylated Human CD3E&CD3D, hFc Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 25.5ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-hFc-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Biotinylated Human CD3E&CD3D, hFc Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 25.5ng/ml determined by ELISA. |
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The purity of Biotinylated Human CD3E&CD3D was greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human CD3E&CD3D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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