Name: Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-HM505B)
Brand: KactusBio
SKU: CD3-HM505B-100UG
Price: 660.0 USD
Availability: InStock

Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-HM505B)

Anti-CD3 Antibody, hFc Tag immobilized on CM5 Chip can bind Biotinylated Human CD3E&CD3D, hFc-Avi Tag with an affinity constant of 0.17 µM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Human CD3E&CD3D, hFc Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 42.1ng/ml determined by ELISA (QC Test).

Immobilized Anti-CD3 Anitibody, hFc Tag at 5ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human CD3E&CD3D, hFc-Avi Tag with the EC50 of 0.12ug/ml determined by ELISA.

Biotinylated Human CD3E&CD3D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human CD3E&CD3D was greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human CD3E&CD3D, hFc Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 42.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.17 µM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D)

Catalog #CD3-HM505B

Size

100UG

500UG

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Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-HM505B) Summary

Product Description	Recombinant Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus of CD3E and hFc tag at the C-Terminus of CD3D.It contains Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D).
Product Background	T-cell surface glycoprotein CD3 epsilon&CD3 delta chain, also known as CD3E&CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (CD3-HM505B) Specifications/Details

Protein	CD3E&CD3D/CD3 epsilon&CD3 delta
Synonyms	CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon
Accession	P07766(CD3E)&P04234(CD3D)
Species	Human
Biotinylated	yes
Amino Acid Range	Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D)
Molecular Weight	The protein has a predicted MW of 40 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation,the protein migrates to 48-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human CD3E&CD3D, hFc Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 42.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.17 µM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Anti-CD3 Antibody, hFc Tag immobilized on CM5 Chip can bind Biotinylated Human CD3E&CD3D, hFc-Avi Tag with an affinity constant of 0.17 µM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human CD3E&CD3D, hFc Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 42.1ng/ml determined by ELISA (QC Test).
	Immobilized Anti-CD3 Anitibody, hFc Tag at 5ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human CD3E&CD3D, hFc-Avi Tag with the EC50 of 0.12ug/ml determined by ELISA.
	Biotinylated Human CD3E&CD3D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human CD3E&CD3D was greater than 95% as determined by SEC-HPLC.