Product Description |
Recombinant Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D). |
Product Background |
T-cell surface glycoprotein CD3 epsilon&CD3 delta chain, also known as CD3E&CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. |
Product Category |
Recombinant Protein |
Target |
CD3E&CD3D/CD3 epsilon&CD3 delta |
Synonyms |
CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Asp23-Asp126(CD3E) & Phe22-Ala105(CD3D) |
Molecular Weight |
The protein has a predicted MW of 40 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation,the protein migrates to 48-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human CD3E&CD3D, hFc Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 42.1ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Biotinylated Human CD3E&CD3D, hFc Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 42.1ng/ml determined by ELISA. |
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Biotinylated Human CD3E&CD3D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human CD3E&CD3D was greater than 95% as determined by SEC-HPLC. |
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