Biotinylated Human CD79B Protein (CD7-HM49BB)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-CD79B Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD79B, His Tag with the EC50 of 11.5ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ala29-Asp159

Catalog: CD7-HM49BB

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Product Description Recombinant Biotinylated Human CD79B Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala29-Asp159.

Product Background

CD79B (also known as B29, Ig beta and B cell antigen receptor complex-associated protein beta-chain) is a 36-40 kDa member of the Ig-Superfamily. It is required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation.

Product Category

Recombinant Protein

Target

CD79B
Synonyms B29; CD79b molecule; CD79B; IGB; IGBAGM6; Ig-beta
Accession

Species

Human

Exact Sequence

Ala29-Asp159
Molecular Weight The protein has a predicted MW of 18.1 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Anti-CD79B Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD79B, His Tag with the EC50 of 11.5ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-CD79B Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD79B, His Tag with the EC50 of 11.5ng/ml determined by ELISA.
The purity of Biotinylated CD79B is greater than 95% as determined by SEC-HPLC.
Biotinylated CD79B on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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