Name: Biotinylated Human EGFR/HER1 Protein (EGF-HM401B)
Brand: KactusBio
SKU: EGF-HM401B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human EGFR/HER1 Protein (EGF-HM401B)

Biotinylated Human EGFR, His Tag captured on CM5 Chip via Anti-his antibody can bind Human EGF, No Tag with an affinity constant of 0.62 nM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Human EGFR, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Human EGF, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test).

Biotinylated Human EGFR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human EGFR is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human EGFR, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Human EGF, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.62 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Leu25-Ser645

Catalog #EGF-HM401B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human EGFR/HER1 Protein (EGF-HM401B) Summary

Product Description	Recombinant Biotinylated Human EGFR/HER1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu25-Ser645.
Product Background	The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Biotinylated Human EGFR/HER1 Protein (EGF-HM401B) Specifications/Details

Protein	EGFR/HER1
Synonyms	ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2
Accession	P00533-1
Species	Human
Biotinylated	yes
Amino Acid Range	Leu25-Ser645
Molecular Weight	The protein has a predicted MW of 71.5 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human EGFR, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Human EGF, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.62 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Biotinylated Human EGFR, His Tag captured on CM5 Chip via Anti-his antibody can bind Human EGF, No Tag with an affinity constant of 0.62 nM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human EGFR, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Human EGF, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test).
	Biotinylated Human EGFR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human EGFR is greater than 95% as determined by SEC-HPLC.