Biotinylated Human EGFR/HER1 Protein (EGF-HM401B)
Biotinylated Human EGFR/HER1 Protein (EGF-HM401B)
Biotinylated Human EGFR/HER1 Protein (EGF-HM401B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human EGFR, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Human EGF, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.62 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Leu25-Ser645
Catalog #EGF-HM401B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human EGFR/HER1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu25-Ser645.
Product Background
The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.
Product Category
Recombinant Protein
Target
EGFR/HER1
Synonyms
ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Leu25-Ser645
Molecular Weight
The protein has a predicted MW of 71.5 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human EGFR, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Human EGF, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.62 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Human EGFR, His Tag captured on CM5 Chip via Anti-his antibody can bind Human EGF, No Tag with an affinity constant of 0.62 nM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human EGFR, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Human EGF, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test).
Biotinylated Human EGFR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human EGFR is greater than 95% as determined by SEC-HPLC.

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