Biotinylated Human EGFR/HER1 Protein (EGF-HM401B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human EGFR at 0.5μg/ml (100μl/Well). Dose response curve for Anti-EGFR Ab., hFc Tag with the EC50 of 0.27μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu25-Ser645

Catalog: EGF-HM401B

$657.00
Size:

Order now get it on

Product Description Recombinant Biotinylated Human EGFR/HER1 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Leu25-Ser645.
Product Category Recombinant Protein
Target EGFR/HER1
Synonyms ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2
Gene/Protein ID P00533-1
Species Human
Exact Sequence Leu25-Ser645
Molecular Weight The protein has a predicted MW of 71.5 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Human EGFR at 0.5μg/ml (100μl/Well). Dose response curve for Anti-EGFR Ab., hFc Tag with the EC50 of 0.27μg/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human EGFR at 0.5μg/ml (100μl/Well). Dose response curve for Anti-EGFR Ab., hFc Tag with the EC50 of 0.27μg/ml determined by ELISA.
Immobilized Human EGF, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human EGFR, His Tag with the EC50 of 6.4μg/ml determined by ELISA.
The purity of Biotinylated Human EGFR/HER1 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human EGFR/HER1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

You may also be interested in