Product Description |
Recombinant Biotinylated Human EGFRVIII Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu25-Ser378. |
Product Background |
The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets |
Protein |
EGFRVIII |
Synonyms |
ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Leu25-Ser378 |
Molecular Weight |
The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 60-78 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human EGFRVIII, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-EGFR VIII Anitibody, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Biotinylated Human EGFRVIII, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-EGFR VIII Anitibody, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA (QC Test). |
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Biotinylated Human EGFRVIII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Use Biotinylated Human EGFRVIII, His Tag protein to detect the expression rate of Anti-EGFRVIII-CAR positive cell. 293T cells and anti-EGFRVIII CAR-293T cells were incubated with Biotinylated human EGFR VIII-His Tag. Non-transfected 293T cells and Biotinylated protein control were used as negative control. SA-PE was used to evaluate the binding activity of Biotinylated Human EGFR VIII. |
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The purity of Biotinylated Human EGFRVIII is greater than 95% as determined by SEC-HPLC. |
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