Biotinylated Human EGFRVIII Protein (EG8-HM154B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-EGFR VIII Anitibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human EGFR VIII, His Tag with the EC50 of 56.1ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Exact Sequence: Leu25-Ser378

Catalog: EG8-HM154B

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Product Description Recombinant Biotinylated Human EGFRVIII Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu25-Ser378.

Product Background

The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.

Product Category

Recombinant Protein

Target

EGFRVIII
Synonyms ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2

Gene/Protein ID

NP_001333870.1

Species

Human

Exact Sequence

Leu25-Ser378
Molecular Weight The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 60-78 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Anti-EGFR VIII Anitibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human EGFR VIII, His Tag with the EC50 of 56.1ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-EGFR VIII Anitibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human EGFR VIII, His Tag with the EC50 of 56.1ng/ml determined by ELISA (QC Test).
Use Biotinylated Human EGFRVIII, His Tag protein to detect the expression rate of Anti-EGFRVIII-CAR positive cell. 293T cells and anti-EGFRVIII CAR-293T cells were incubated with Biotinylated human EGFR VIII-His Tag. Non-transfected 293T cells and Biotinylated protein control were used as negative control. SA-PE was used to evaluate the binding activity of Biotinylated Human EGFR VIII.
The purity of Biotinylated Human EGFRVIII is greater than 95% as determined by SEC-HPLC.
Biotinylated Human EGFRVIII on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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