Biotinylated Human EGFRVIII Protein (EG8-HM154B)
Biotinylated Human EGFRVIII Protein (EG8-HM154B)
Biotinylated Human EGFRVIII Protein (EG8-HM154B)
Biotinylated Human EGFRVIII Protein (EG8-HM154B)
Biotinylated Human EGFRVIII Protein (EG8-HM154B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human EGFRVIII, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-EGFR VIII Anitibody, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Leu25-Ser378
Catalog #EG8-HM154B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human EGFRVIII Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu25-Ser378.
Product Background
The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.
Product Category
Recombinant Protein
Target
EGFRVIII
Synonyms
ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Leu25-Ser378
Molecular Weight
The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 60-78 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human EGFRVIII, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-EGFR VIII Anitibody, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human EGFRVIII, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-EGFR VIII Anitibody, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA (QC Test).
Biotinylated Human EGFRVIII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Use Biotinylated Human EGFRVIII, His Tag protein to detect the expression rate of Anti-EGFRVIII-CAR positive cell. 293T cells and anti-EGFRVIII CAR-293T cells were incubated with Biotinylated human EGFR VIII-His Tag. Non-transfected 293T cells and Biotinylated protein control were used as negative control. SA-PE was used to evaluate the binding activity of Biotinylated Human EGFR VIII.
The purity of Biotinylated Human EGFRVIII is greater than 95% as determined by SEC-HPLC.

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