Biotinylated Human EGFRVIII Protein (EG8-HM154B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-EGFR VIII Anitibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human EGFR VIII, His Tag with the EC50 of 36.6ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu25-Ser378

Catalog: EG8-HM154B

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Product Description Recombinant Biotinylated Human EGFRVIII Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Leu25-Ser378.
Product Category Recombinant Protein
Target EGFRVIII
Synonyms ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2
Gene/Protein ID NP_001333870.1
Species Human
Exact Sequence Leu25-Ser378
Molecular Weight The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 65-78 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-EGFR VIII Anitibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human EGFR VIII, His Tag with the EC50 of 36.6ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-EGFR VIII Anitibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human EGFR VIII, His Tag with the EC50 of 36.6ng/ml determined by ELISA.
Use Biotinylated Human EGFRVIII, His Tag protein to detect the expression rate of Anti-EGFRVIII-CAR positive cell. 293T cells and anti-EGFRVIII CAR-293T cells were incubated with Biotinylated human EGFR VIII-His Tag. Non-transfected 293T cells and Biotinylated protein control were used as negative control. SA-PE was used to evaluate the binding activity of Biotinylated Human EGFR VIII.
The purity of Biotinylated Human EGFRVIII is greater than 95% as determined by SEC-HPLC.
Biotinylated Human EGFRVIII on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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