Name: Biotinylated Human ENPP-3 Protein (ENP-HM403B)
Brand: KactusBio
SKU: ENP-HM403B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human ENPP-3 Protein (ENP-HM403B)

Immobilized Biotinylated Human ENPP-3, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ENPP-3 Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA.

Biotinylated Human ENPP-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human ENPP-3 is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Biotinylated Human ENPP-3, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ENPP-3 Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA. See testing image for detail.Measured by its ability to hydrolyze thymidine 5'-monophosphate p-nitrophenyl ester. The specific activity is > 8000 pmol/min/ug.
Amino Acid Range: Leu48-Ile875
Other Available Proteins:

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Catalog #ENP-HM403B

Size

100UG

500UG

25UG

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Biotinylated Human ENPP-3 Protein (ENP-HM403B) Summary

Product Description	Recombinant Biotinylated Human ENPP-3 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Leu48-Ile875.
Product Background	Ectonucleotide pyrophosphatase-phosphodiesterase 3 (ENPP3), a protein detected in the human uterus, has been found to play an important role in the development and invasion of tumours. It was recently discovered that ENPP3 was upregulated during the window of implantation in the human endometrium but its functional relevance remains elusive.
Product Category	Recombinant Protein / Antibody discovery / ADC targets

Biotinylated Human ENPP-3 Protein (ENP-HM403B) Specifications/Details

Protein	ENPP-3
Synonyms	E-NPP 3; NPP3; PD-Ibeta; NPPase; ENPP3; PDNP3; CD203c; B10; gp130RB13-6; PD-Iβ
Accession	O14638
Species	Human
Biotinylated	yes
Amino Acid Range	Leu48-Ile875
Molecular Weight	The protein has a predicted MW of 99.97 kDa. Due to glycosylation, the protein migrates to 110-130 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human ENPP-3, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ENPP-3 Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA. See testing image for detail.Measured by its ability to hydrolyze thymidine 5'-monophosphate p-nitrophenyl ester. The specific activity is > 8000 pmol/min/ug.
Product Tag	N-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22um filtered solution in 50mM Tris, 150mM NaCl (pH 8.0).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human ENPP-3, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ENPP-3 Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA.
	Biotinylated Human ENPP-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human ENPP-3 is greater than 90% as determined by SEC-HPLC.