Biotinylated Human EpCAM/TROP1 Protein (CAM-HM4EPB)
Biotinylated Human EpCAM/TROP1 Protein (CAM-HM4EPB)
Biotinylated Human EpCAM/TROP1 Protein (CAM-HM4EPB)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Anti-EpCAM Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human EpCAM, His Tag with the EC50 of 21.1ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gln24-Lys265
Catalog #CAM-HM4EPB
Regular price $525.00
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Product Description
Recombinant Biotinylated Human EpCAM/TROP1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln24-Lys265.
Product Background
Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.
Product Category
Recombinant Protein
Target
EpCAM/TROP1
Synonyms
CD326; EGP; EGP-2; EGP314; EGP40; EpCAM; 17-1A; 323/A3; ACSTD1; DIAR5; GA733-2; gp40; HNPCC8; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; ESA; KS1,4; KSA; MIC18; MK1
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Gln24-Lys265
Molecular Weight
The protein has a predicted MW of 30.1 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Anti-EpCAM Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human EpCAM, His Tag with the EC50 of 21.1ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-EpCAM Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human EpCAM, His Tag with the EC50 of 21.1ng/ml determined by ELISA (QC Test).
Biotinylated Human EpCAM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%..
The purity of Biotinylated Human EpCAM is greater than 95% as determined by SEC-HPLC.

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