Biotinylated Human EpCAM/TROP1 Protein (CAM-HM4EPB)
Biotinylated Human EpCAM/TROP1 Protein (CAM-HM4EPB)
Biotinylated Human EpCAM/TROP1 Protein (CAM-HM4EPB)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Anti-EpCAM Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human EpCAM, His Tag with the EC50 of 21.1ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gln24-Lys265
Catalog #CAM-HM4EPB
Regular price $577.50
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Product Description

Recombinant Biotinylated Human EpCAM/TROP1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln24-Lys265.

Product Background

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.

Product Category

Recombinant Protein / Antibody discovery / CAR-T targets

Protein

EpCAM/TROP1

Synonyms

CD326; EGP; EGP-2; EGP314; EGP40; EpCAM; ACSTD1; DIAR5; gp40; HNPCC8; MOC31; TACST-1; TACSTD1; TROP1; ESA; KSA; MIC18; MK1

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Gln24-Lys265

Molecular Weight

The protein has a predicted MW of 30.1 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Anti-EpCAM Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human EpCAM, His Tag with the EC50 of 21.1ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Anti-EpCAM Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human EpCAM, His Tag with the EC50 of 21.1ng/ml determined by ELISA (QC Test). Immobilized Anti-EpCAM Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human EpCAM, His Tag with the EC50 of 21.1ng/ml determined by ELISA (QC Test).
Biotinylated Human EpCAM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.. Biotinylated Human EpCAM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%..
The purity of Biotinylated Human EpCAM is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human EpCAM is greater than 95% as determined by SEC-HPLC.

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support@kactusbio.us

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