Biotinylated Human EpCAM/TROP1 Protein (CAM-HM4EPB)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human Trop1, His Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Trop1 Ab., hFc Tag with the EC50 of 15.6ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln24-Lys245

Catalog: CAM-HM4EPB

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Product Description Recombinant Biotinylated Human EpCAM/TROP1 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Gln24-Lys245.
Product Category Recombinant Protein
Target EpCAM/TROP1
Synonyms CD326; EGP; EGP-2; EGP314; EGP40; EpCAM; 17-1A; 323/A3; ACSTD1; DIAR5; GA733-2; gp40; HNPCC8; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; ESA; KS1, 4; KSA; MIC18; MK1
Gene/Protein ID P16422
Species Human
Exact Sequence Gln24-Lys245
Molecular Weight The protein has a predicted MW of 30.1 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Human Trop1, His Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Trop1 Ab., hFc Tag with the EC50 of 15.6ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human Trop1, His Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Trop1 Ab., hFc Tag with the EC50 of 15.6ng/ml determined by ELISA.
The purity of Biotinylated Human EpCAM is greater than 95% as determined by SEC-HPLC.
Biotinylated Human EpCAM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%..

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