Product Description |
Recombinant Biotinylated Human Fc gamma RIIIB/CD16b (NA2) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly17-Ser200(NA2). The CD16b NA1 differ with the CD16b NA2 in AA36, 65, 82, and 106. The CD16b NA1form carries R36, N65, D82, and V106, while the CD16b NA2 form carries S36, S65, N82, and I106. |
Product Background |
Human Fc gamma RIIIB/CD16b Protein is a receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils. |
Product Category |
Recombinant Protein |
Target |
Fc gamma RIIIB/CD16b (NA2) |
Synonyms |
CD16; Fc gamma RIIIB; FCG3; FCGR3B; FcgRIIIB; FCRIIIB; IGFR3; CD16b (NA2); CD16B; FCG3B; FCG3; FCGR3; FCR-10 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gly17-Ser200(NA2) |
Molecular Weight |
The protein has a predicted MW of 23.7 kDa. Due to glycosylation, the protein migrates to 47-53 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 3.50 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Biotinylated Human Fc gamma RIIIB (NA2), His Tag captured on CM5 Chip via Anti-his antibody can bind Rituximab with an affinity constant of 3.50 uM as determined in SPR assay (Biacore T200). |
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Biotinylated Human Fc gamma RIIIB (NA2) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human Fc gamma RIIIB (NA2) is greater than 95% as determined by SEC-HPLC. |
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