Biotinylated Human Fc gamma RIIIB/CD16b (NA2) Protein (FCR-HM42BB)
Catalog: FCR-HM42BB
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| Product Description | Recombinant Biotinylated Human Fc gamma RIIIB/CD16b (NA2) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly17-Ser200(NA2). The CD16b NA1 differ with the CD16b NA2 in AA36, 65, 82, and 106. The CD16b NA1form carries R36, N65, D82, and V106, while the CD16b NA2 form carries S36, S65, N82, and I106. |
| Product Category | Recombinant Protein |
| Target | Fc gamma RIIIB/CD16b (NA2) |
| Synonyms | CD16; Fc gamma RIIIB; FCG3; Fc-gamma receptor IIIb (CD 16); Fc-gamma RIIIb; Fc-gamma RIII-beta; FCGR3B; FcgRIIIB; FCRIIIB; IGFR3; IgG Fc receptor III-1; CD16b (NA2); CD16B; FCG3B; FCG3; FCGR3; FCR-10 |
| Gene/Protein ID | O75015-1 |
| Species | Human |
| Exact Sequence | Gly17-Ser200(NA2) |
| Molecular Weight | The protein has a predicted MW of 23.7 kDa. Due to glycosylation, the protein migrates to 47-53 kDa based on Tris-Bis PAGE result. |
| Activity | The affinity constant of 4.3uM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Biotinylated Human Fc gamma RIIIB (NA2) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab, hFc Tag with an affinity constant of 4.3uM as determined in SPR assay (Biacore T200). |
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The purity of Biotinylated Human Fc gamma RIIIB (NA2) is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human Fc gamma RIIIB (NA2) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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