Name: Biotinylated Human FOLR1 Protein (FOL-HM4R1B)
Brand: KactusBio
SKU: FOL-HM4R1B-100UG
Price: 577.5 USD
Availability: InStock

ELISA binding curve for biotinylated human FOLR1 protein, showing OD450 absorbance vs. log antibody concentration (µg/ml).

Biotinylated Human FOLR1 Protein (FOL-HM4R1B)

Immobilized Biotinylated Human FOLR1, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-FOLR1 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA.

Biotinylated Human FOLR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Anti-FOLR1 Ab., hFc-Avi Tag on ProA-Biosensor can bind Biotinylated Human FOLR1, His-Avi Tag with an affinity constant of 0.42 nM as determined in BLI assay (Gator® Prime).

The purity of Biotinylated Human FOLR1 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human FOLR1, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-FOLR1 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Arg25-Met233

Catalog #FOL-HM4R1B

Size

100UG

500UG

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Biotinylated Human FOLR1 Protein (FOL-HM4R1B) Summary

Product Description	Recombinant Biotinylated Human FOLR1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg25-Met233.
Product Background	Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37 - 42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown. FOLR1 binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Biotinylated Human FOLR1 Protein (FOL-HM4R1B) Specifications/Details

Protein	FOLR1
Synonyms	FR-alpha; FR alpha; FR α; FOLR; FOLR1; FBP; Folbp1; KB cells FBP; MOv18; Folate receptor 1; Fbp1; Folate receptor alpha;
Accession	P15328
Species	Human
Biotinylated	yes
Amino Acid Range	Arg25-Met233
Molecular Weight	The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human FOLR1, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-FOLR1 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human FOLR1, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-FOLR1 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA.
	Biotinylated Human FOLR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Anti-FOLR1 Ab., hFc-Avi Tag on ProA-Biosensor can bind Biotinylated Human FOLR1, His-Avi Tag with an affinity constant of 0.42 nM as determined in BLI assay (Gator® Prime).
	The purity of Biotinylated Human FOLR1 is greater than 95% as determined by SEC-HPLC.