Biotinylated Human GPC3/Glypican 3 Protein (GPC-HM431B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized Anti-GPC3 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GPC3, His Tag with the EC50 of 10.8ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln25-His559

Catalog: GPC-HM431B

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Product Description Recombinant Biotinylated Human GPC3/Glypican 3 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Gln25-His559.
Product Category Recombinant Protein
Target GPC3/Glypican 3
Synonyms GPC3; DGSX; Glypican 3; GTR2-2; MXR7; OCI5; OCI-5; SGB; SGBS; SGBS1SDYS; SDYS; SGBS1
Gene/Protein ID P51654-1
Species Human
Exact Sequence Gln25-His559
Molecular Weight The protein has a predicted MW of 63.6 kDa. Due to glycosylation, the protein migrates to 42 kDa, 80-135 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-GPC3 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GPC3, His Tag with the EC50 of 10.8ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-GPC3 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GPC3, His Tag with the EC50 of 10.8ng/ml determined by ELISA.
Use Biotinylated Human GPC3-His protein to detect the expression rate of Anti-GPC3-CAR positive cells. 293T cells and anti-GPC3 CAR-293T cells were incubated with Biotinylated human GPC3-His (25-559), His Tag and Biotinylated Protein Control-His. Non-transfected 293 cells and Biotinylated Protein Control-His were used as negative control. SA-FITC was used to evaluate the binding activity of Biotinylated Human GPC3.
Biotinylated Human GPC3 on Tris-Bis PAGE under reduced condition. The purity is greater than 90%.

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