Biotinylated Human GPC3/Glypican 3 Protein (GPC-HM431B)
Catalog: GPC-HM431B
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| Product Description | Recombinant Biotinylated Human GPC3/Glypican 3 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Gln25-His559. |
| Product Category | Recombinant Protein |
| Target | GPC3/Glypican 3 |
| Synonyms | GPC3; DGSX; Glypican 3; GTR2-2; MXR7; OCI5; OCI-5; SGB; SGBS; SGBS1SDYS; SDYS; SGBS1 |
| Gene/Protein ID | P51654-1 |
| Species | Human |
| Exact Sequence | Gln25-His559 |
| Molecular Weight | The protein has a predicted MW of 63.6 kDa. Due to glycosylation, the protein migrates to 42 kDa, 68-80 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Anti-GPC3 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GPC3, His Tag with the EC50 of 10.8ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Anti-GPC3 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GPC3, His Tag with the EC50 of 10.8ng/ml determined by ELISA. |
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Use Biotinylated Human GPC3-His protein to detect the expression rate of Anti-GPC3-CAR positive cells. 293T cells and anti-GPC3 CAR-293T cells were incubated with Biotinylated human GPC3-His (25-559) , His Tag and Biotinylated Protein Control-His. Non-transfected 293 cells and Biotinylated Protein Control-His were used as negative control. SA-FITC was used to evaluate the binding activity of Biotinylated Human GPC3. |
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The purity of Biotinylated Human GPC3 is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human GPC3 on Tris-Bis PAGE under reduced condition. The purity is greater than 90%. |
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