Product Description |
Recombinant Biotinylated Human GPC3/Glypican 3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln25-His559. |
Product Background |
Glypican-3 is a protein ,which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets |
Protein |
GPC3/Glypican 3 |
Synonyms |
GPC3; DGSX; Glypican 3; GTR2-2; MXR7; OCI5; OCI-5; SGB; SGBS; SGBS1SDYS; SDYS; SGBS1 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gln25-His559 |
Molecular Weight |
The protein has a predicted MW of 63.6 kDa. Due to glycosylation, the protein migrates to 42 kDa, 68-80 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human GPC3, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Biotinylated Human GPC3, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA (QC Test). |
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Biotinylated Human GPC3 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. |
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Use Biotinylated Human GPC3-His protein to detect the expression rate of Anti-GPC3-CAR positive cells. 293T cells and anti-GPC3 CAR-293T cells were incubated with Biotinylated human GPC3-His (25-559) , His Tag and Biotinylated Protein Control-His. Non-transfected 293 cells and Biotinylated Protein Control-His were used as negative control. SA-FITC was used to evaluate the binding activity of Biotinylated Human GPC3. |
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The purity of Biotinylated Human GPC3 is greater than 95% as determined by SEC-HPLC. |
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