Biotinylated Human GUCY2C/Guanylyl cyclase C Protein (GCC-HM401B)
Biotinylated Human GUCY2C/Guanylyl cyclase C Protein (GCC-HM401B)
Biotinylated Human GUCY2C/Guanylyl cyclase C Protein (GCC-HM401B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Anti-GUCY2C Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GUCY2C, His Tag with the EC50 of 35.3ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ser24-Gln430
Catalog #GCC-HM401B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human GUCY2C/Guanylyl cyclase C Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser24-Gln430.
Product Background
Guanylyl cyclase C (GUCY2C) has canonical centrality in defense of key intestinal homeostatic mechanisms, encompassing fluid and electrolyte balance, epithelial dynamics, antitumorigenesis, and intestinal barrier function. GUCY2C may represent a new target for anti-obesity pharmacotherapy.
Product Category
Recombinant Protein
Target
GUCY2C/Guanylyl cyclase C
Synonyms
Guanylyl cyclase C; GC-C; STAR; GUCY2C; GUC2C; STA receptor; DIAR6; EC 4.6.1; GCC; GUC2CEC 4.6.1.2; MUCIL
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Ser24-Gln430
Molecular Weight
The protein has a predicted MW of 48.8 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Anti-GUCY2C Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GUCY2C, His Tag with the EC50 of 35.3ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-GUCY2C Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GUCY2C, His Tag with the EC50 of 35.3ng/ml determined by ELISA.
Biotinylated Human GUCY2C on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human GUCY2C is greater than 95% as determined by SEC-HPLC.
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