Biotinylated Human GUCY2C/Guanylyl cyclase C Protein (GCC-HM401B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-GUCY2C Antibody at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GUCY2C, His Tag with the EC50 of 24.1ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ser24-Gln430

Catalog: GCC-HM401B

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Product Description Recombinant Biotinylated Human GUCY2C/Guanylyl cyclase C Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Ser24-Gln430.
Product Category Recombinant Protein
Target GUCY2C/Guanylyl cyclase C
Synonyms Guanylyl cyclase C; GC-C; STAR; GUCY2C; GUC2C; STA receptor; DIAR6; EC 4.6.1; GCC; GUC2CEC 4.6.1.2; MUCIL
Gene/Protein ID P25092-1
Species Human
Exact Sequence Ser24-Gln430
Molecular Weight The protein has a predicted MW of 48.8 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-GUCY2C Antibody at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GUCY2C, His Tag with the EC50 of 24.1ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-GUCY2C Antibody at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GUCY2C, His Tag with the EC50 of 24.1ng/ml determined by ELISA.
The purity of Biotinylated Human GUCY2C is greater than 95% as determined by SEC-HPLC.
Biotinylated Human GUCY2C on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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