Biotinylated Human Her2/ErbB2 Domain 4 Protein (HER-HM404B)
Biotinylated Human Her2/ErbB2 Domain 4 Protein (HER-HM404B)
Biotinylated Human Her2/ErbB2 Domain 4 Protein (HER-HM404B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human Her2 Domain 4, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HER2 Antibody, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Pro489-Cys630
Catalog #HER-HM404B
Regular price $525.00
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Product Description

Recombinant Biotinylated Human Her2/ErbB2 Domain 4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Pro489-Cys630.

Product Background

ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors.Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane.

Product Category

Recombinant Protein / Antibody discovery / CAR-T targets

Protein

Her2/ErbB2 Domain 4

Synonyms

CD340; EGFR2; ErbB2; HER2; HER-2; herstatin; MLN 19; MLN19; NGL; TKR1; ERBB2; NEU; ENV; ENVW; ERVWE1; HERV7Q; HERVW

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Pro489-Cys630

Molecular Weight

The protein has a predicted MW of 18.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated Human Her2 Domain 4, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HER2 Antibody, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human Her2 Domain 4, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HER2 Antibody, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA. Immobilized Biotinylated Human Her2 Domain 4, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HER2 Antibody, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA.
Biotinylated Human Her2 Domain 4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human Her2 Domain 4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Her2 Domain 4 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human Her2 Domain 4 is greater than 95% as determined by SEC-HPLC.

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