Biotinylated Human Her2/ErbB2 Domain 4 Protein (HER-HM404B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-HER2 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Her2 Domain 4, His Tag with the EC50 of 0.82ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Pro489-Cys630

Catalog: HER-HM404B

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Product Description Recombinant Biotinylated Human Her2/ErbB2 Domain 4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Pro489-Cys630.
Product Category Recombinant Protein
Target Her2/ErbB2 Domain 4
Gene/Protein ID P04626-1
Species Human
Exact Sequence Pro489-Cys630
Molecular Weight The protein has a predicted MW of 18.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-HER2 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Her2 Domain 4, His Tag with the EC50 of 0.82ug/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-HER2 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Her2 Domain 4, His Tag with the EC50 of 0.82ug/ml determined by ELISA.
The purity of Biotinylated Human Her2 Domain 4 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human Her2 Domain 4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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