Product Description |
Recombinant Biotinylated Human Her3/ErbB3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.; It contains Ser20-Thr643. |
Product Background |
Her3, also called ErbB3, is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors.Her3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric Her3 serves as a low affinity receptor for the heregulins (HRG).ation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand. |
Product Category |
Recombinant Protein |
Target |
Her3/ErbB3 |
Synonyms |
ErbB3; ErbB-3; HER3; HER3c-erbB-3; LCCS2; MDA-BF-1; MGC88033; p180-ErbB3; p45-sErbB3; p85-sErbB3; ERBB3 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ser20-Thr643 |
Molecular Weight |
The protein has a predicted MW of 71.6 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Anti-Her3 Antibody, hFc Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylatd Human Her3, His Tag with the EC50 of 58.1 ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Anti-Her3 Antibody, hFc Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylatd Human Her3, His Tag with the EC50 of 58.1 ng/ml determined by ELISA. |
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Immobilized Biotinylated Human Her3, His Tag at 2 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Human NRG1 Beta 1, hFc Tag with the EC50 of 8.3 ng/ml determined by ELISA (QC Test). |
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Biotinylated Human Her3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human Her3 is greater than 95% as determined by SEC-HPLC. |
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