Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Monomer Protein (HLG-HM41CB)
Catalog: HLG-HM41CB
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| Product Description | Recombinant Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-G),Ile21-Met119(B2M) and RIIPRHLQL peptide. |
| Product Category | Recombinant Protein |
| Target | HLA-G&B2M&Peptide (RIIPRHLQL) Monomer |
| Synonyms | HLA G antigen; sHLA-G; b2 microglobulin; HLA G; HLAG; HLA-G; MHC Class I Antigen G; MHC class Ib antigen; MHC-G; sHLA-G |
| Gene/Protein ID | P17693-1(HLA-G)&P61769(B2M)&RIIPRHLQL |
| Species | Human |
| Exact Sequence | Gly25-Thr305(HLA-G),Ile21-Met119(B2M)and RIIPRHLQL peptide |
| Molecular Weight | The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 51-60 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Anti-HLA-G Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Monomer, His Tag with the EC50 of 36.5ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4.). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Anti-HLA-G Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Monomer, His Tag with the EC50 of 36.5ng/ml determined by ELISA. |
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The purity of Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Monomer is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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