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Biotinylated Human IGF1R/CD221 Protein (IGF-HM41RB) Summary
Product Description |
Recombinant Biotinylated Human IGF1R/CD221 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu31-Asn932. |
Product Background |
The type 1 IGF receptor (IGF1R) is a transmembrane tyrosine kinase that is frequently overexpressed by tumours, and mediates proliferation and apoptosis protection. IGF signalling also influences hypoxia signalling, protease secretion, tumour cell motility and adhesion, and thus can affect the propensity for invasion and metastasis. Therefore, the IGF1R is now an attractive anti-cancer treatment target. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Biotinylated Human IGF1R/CD221 Protein (IGF-HM41RB) Specifications/Details
Protein |
IGF1R/CD221 |
Synonyms |
CD221; IGF1R; IGF-1R; IGF-I R; IGF-I receptor; IGFIR; IGFR; JTK13; MGC142170; MGC142172; MGC18216 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Glu31-Asn932 |
Molecular Weight |
The protein has a predicted MW of 80.4 kDa (alpha subunit) and 25.4 kDa (beta subunit). Due to the furin cleavage site and glycosylation, the protein migrates to 110-120 kDa and 52-55 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human IGF1R, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IGF-I, hFc Tag with the EC50 of 1.13ug/ml determined by ELISA (QC Test). |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human IGF1R, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IGF-I, hFc Tag with the EC50 of 1.13ug/ml determined by ELISA (QC Test). |
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Immobilized Biotinylated Human IGF1R, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-IGF1R Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA. |
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Biotinylated Human IGF1R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human IGF1R is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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