Name: Biotinylated Human IL-13 Protein (IL1-HM413B)
Brand: KactusBio
SKU: IL1-HM413B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human IL-13 Protein (IL1-HM413B)

Human IL-13Ra1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human IL-13, His Tag with an affinity constant of 25.59 nM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Human IL-13, His-Avi Tag at 5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human IL-13Ra2, hFc Tag with the EC50 of 0.81ug/ml determined by ELISA (QC Test).

Biotinylated IL-13 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

The purity of Biotinylated Human IL-13 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His-Avi
Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Activity: Immobilized Biotinylated Human IL-13, His-Avi Tag at 5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human IL-13Ra2, hFc Tag with the EC50 of 0.81ug/ml determined by ELISA (QC Test). The affinity constant of 25.59 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gly21-Asn132

Catalog #IL1-HM413B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human IL-13 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Gly21-Asn132.
Product Background	Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).
Product Category	Recombinant Protein / Antibody discovery

Protein	IL-13
Synonyms	IL13; IL-13; interleukin 13; NC30; P600; ALRH; BHR1; MGC116786; MGC116788; MGC116789
Accession	AAK53823
Species	Human
Biotinylated	yes
Amino Acid Range	Gly21-Asn132
Molecular Weight	The protein has a predicted MW of 15.3 kDa. Due to glycosylation, the protein migrates to 15 kDa and 35-45 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human IL-13, His-Avi Tag at 5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human IL-13Ra2, hFc Tag with the EC50 of 0.81ug/ml determined by ELISA (QC Test). The affinity constant of 25.59 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His-Avi
Expression System	HEK293
Purity	>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human IL-13Ra1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human IL-13, His Tag with an affinity constant of 25.59 nM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human IL-13, His-Avi Tag at 5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human IL-13Ra2, hFc Tag with the EC50 of 0.81ug/ml determined by ELISA (QC Test).
	Biotinylated IL-13 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
	The purity of Biotinylated Human IL-13 is greater than 95% as determined by SEC-HPLC.