Biotinylated Human IL-13 Protein (IL1-HM413B)
Biotinylated Human IL-13 Protein (IL1-HM413B)
Biotinylated Human IL-13 Protein (IL1-HM413B)
Biotinylated Human IL-13 Protein (IL1-HM413B)
  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human IL-13, His-Avi Tag at 5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human IL-13Ra2, hFc Tag with the EC50 of 0.81ug/ml determined by ELISA (QC Test). The affinity constant of 25.59 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly21-Asn132
Catalog #IL1-HM413B
Regular price $577.50
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Product Description

Recombinant Biotinylated Human IL-13 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Gly21-Asn132.

Product Background

Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

IL-13

Synonyms

IL13; IL-13; interleukin 13; NC30; P600; ALRH; BHR1; MGC116786; MGC116788; MGC116789

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Gly21-Asn132

Molecular Weight

The protein has a predicted MW of 15.3 kDa. Due to glycosylation, the protein migrates to 15 kDa and 35-45 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated Human IL-13, His-Avi Tag at 5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human IL-13Ra2, hFc Tag with the EC50 of 0.81ug/ml determined by ELISA (QC Test). The affinity constant of 25.59 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

N-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human IL-13Ra1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human IL-13, His Tag with an affinity constant of 25.59 nM as determined in SPR assay (Biacore T200). Human IL-13Ra1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human IL-13, His Tag with an affinity constant of 25.59 nM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human IL-13, His-Avi Tag at 5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human IL-13Ra2, hFc Tag with the EC50 of 0.81ug/ml determined by ELISA (QC Test). Immobilized Biotinylated Human IL-13, His-Avi Tag at 5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human IL-13Ra2, hFc Tag with the EC50 of 0.81ug/ml determined by ELISA (QC Test).
Biotinylated IL-13 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Biotinylated IL-13 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Biotinylated Human IL-13 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human IL-13 is greater than 95% as determined by SEC-HPLC.

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