Biotinylated Human IL-13 Protein (IL1-HM413B)

  • Express System: Expi293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human IL-13, His Tag at 2μg/ml (100μl/well) on the streptavidin (5μg/ml) precoated plate. Dose response curve for Human IL-13Ra2, hFc Tag with the EC50 of 1.4μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gly21-Asn132

Catalog: IL1-HM413B

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Product Description Recombinant Biotinylated Human IL-13 Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal.It contains Gly21-Asn132.
Product Category Recombinant Protein
Target IL-13
Synonyms IL13; IL-13; interleukin 13; NC30; P600; ALRH; BHR1; MGC116786; MGC116788; MGC116789
Gene/Protein ID AAK53823
Species Human
Exact Sequence Gly21-Asn132
Molecular Weight The protein has a predicted MW of 15.3 kDa. Due to glycosylation, the protein migrates to 15 kDa and 35-45 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Human IL-13, His Tag at 2μg/ml (100μl/well) on the streptavidin (5μg/ml) precoated plate. Dose response curve for Human IL-13Ra2, hFc Tag with the EC50 of 1.4μg/ml determined by ELISA. See testing image for detail.
Product Tag N-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human IL-13, His Tag at 2μg/ml (100μl/well) on the streptavidin (5μg/ml) precoated plate. Dose response curve for Human IL-13Ra2, hFc Tag with the EC50 of 1.4μg/ml determined by ELISA.
Biotinylated IL-13 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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