Name: Biotinylated Human IL-15RA&IL-15 Protein (Primary Amine Labeling) (IL1-HM1R5B)
Brand: KactusBio
SKU: IL1-HM1R5B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human IL-15RA&IL-15 Protein (Primary Amine Labeling) (IL1-HM1R5B)

Express System: HEK293
Product Tag: C-His
Purity: >95% as determined by Bis-Tris PAGE
Activity: Immobilized Biotinylated Human IL-15Ra&IL-15, His Tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Human IL-2 R beta, hFc Tag with the EC50 of 7.8 ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Ile31-Ser108 (IL-15RA) & Asn49-Ser162 (IL-15)

Catalog #IL1-HM1R5B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human IL-15RA&IL-15 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.; It contains Ile31-Ser108 (IL-15RA)&Asn49-Ser162 (IL-15).
Product Background	Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development.
Product Category	Recombinant Protein

Target	IL-15RA&IL-15
Synonyms	IL-15 R alpha；CD215；IL15RA；IL-15RA；IL-15R-alpha1；Interleukin-15；IL-15；IL15
Accession	Q13261-1 (IL-15RA)&P40933-1 (IL-15)
Species	Human
Biotinylated	yes
Amino Acid Range	Ile31-Ser108 (IL-15RA) & Asn49-Ser162 (IL-15)
Molecular Weight	The protein has a predicted MW of 23.8 kDa. Due to glycosylation, the protein migrates to 25-28 kDa&30-35 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human IL-15Ra&IL-15, His Tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Human IL-2 R beta, hFc Tag with the EC50 of 7.8 ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	>95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Biotinylated Human IL-15Ra&IL-15, His Tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Human IL-2 R beta, hFc Tag with the EC50 of 7.8 ng/ml determined by ELISA.
	Biotinylated Human IL-15RA&IL-15 (Primary Amine Labeling) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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