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Biotinylated Human IL-15RA&IL-15 Protein (Primary Amine Labeling) (IL1-HM1R5B) Summary
Product Description |
Recombinant Biotinylated Human IL-15RA&IL-15 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.; It contains Ile31-Ser108 (IL-15RA)&Asn49-Ser162 (IL-15). |
Product Background |
Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Biotinylated Human IL-15RA&IL-15 Protein (Primary Amine Labeling) (IL1-HM1R5B) Specifications/Details
Protein |
IL-15RA&IL-15 |
Synonyms |
IL-15 R alpha;CD215;IL15RA;IL-15RA;IL-15R-alpha1;Interleukin-15;IL-15;IL15; Nanrilkefusp alfa |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ile31-Ser108 (IL-15RA) & Asn49-Ser162 (IL-15) |
Molecular Weight |
The protein has a predicted MW of 23.8 kDa. Due to glycosylation, the protein migrates to 25-28 kDa&30-35 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human IL-15Ra&IL-15, His Tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Human IL-2 R beta, hFc Tag with the EC50 of 7.8 ng/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human IL-15Ra&IL-15, His Tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Human IL-2 R beta, hFc Tag with the EC50 of 7.8 ng/ml determined by ELISA. |
|
|
Biotinylated Human IL-15RA&IL-15 (Primary Amine Labeling) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
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