• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Biotinylated Human IL-15Ra&IL-15, His Tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Human IL-2 R beta, hFc Tag with the EC50 of 7.8 ng/ml determined by ELISA.
  • Amino Acid Range: Ile31-Ser108 (IL-15RA) & Asn49-Ser162 (IL-15)
Catalog #IL1-HM1R5B
Price
Regular price $57750
Regular price Sale price $57750
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human IL-15RA&IL-15 Protein (Primary Amine Labeling) (IL1-HM1R5B) Summary

Product Description

 Recombinant Biotinylated Human IL-15RA&IL-15 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.; It contains Ile31-Ser108 (IL-15RA)&Asn49-Ser162 (IL-15).

Product Background

 Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development.

Biotinylated Human IL-15RA&IL-15 Protein (Primary Amine Labeling) (IL1-HM1R5B) Specifications/Details

Protein
 IL-15RA&IL-15
Synonyms
 IL-15 R alpha;CD215;IL15RA;IL-15RA;IL-15R-alpha1;Interleukin-15;IL-15;IL15; Nanrilkefusp alfa;IL15R alpha; IL15R-alpha1;IL 15; Interleukin 15
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Ile31-Ser108 (IL-15RA) & Asn49-Ser162 (IL-15)
Molecular Weight
 The protein has a predicted MW of 23.8 kDa. Due to glycosylation, the protein migrates to 25-28 kDa&30-35 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human IL-15Ra&IL-15, His Tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Human IL-2 R beta, hFc Tag with the EC50 of 7.8 ng/ml determined by ELISA.
Product Tag
 C-His
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human IL-15Ra&IL-15, His Tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Human IL-2 R beta, hFc Tag with the EC50 of 7.8 ng/ml determined by ELISA. Immobilized Biotinylated Human IL-15Ra&IL-15, His Tag at 1 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Human IL-2 R beta, hFc Tag with the EC50 of 7.8 ng/ml determined by ELISA.
Biotinylated Human IL-15RA&IL-15 (Primary Amine Labeling) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human IL-15RA&IL-15 (Primary Amine Labeling) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...