Biotinylated Human IL-15RA&IL-15 Protein (Primary Amine Labeling) (IL1-HM1R5B)
Catalog: IL1-HM1R5B
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Product Description | Recombinant Biotinylated Human IL-15RA&IL-15 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.; It contains Ile31-Ser108 (IL-15RA)&Asn49-Ser162 (IL-15). |
Product Background |
Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development. |
Product Category |
Recombinant Protein |
Target |
IL-15RA&IL-15 |
Synonyms | IL-15 R alpha;CD215;IL15RA;IL-15RA;IL-15R-alpha1;Interleukin-15;IL-15;IL15 |
Accession | |
Species |
Human |
Amino Acid Range |
Ile31-Ser108 (IL-15RA) & Asn49-Ser162 (IL-15) |
Molecular Weight | The protein has a predicted MW of 23.8 kDa. Due to glycosylation, the protein migrates to 25-28 kDa&30-35 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Human IL-2 R beta, hFc Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human IL-15Ra&IL-15, His Tag with the EC50 of 18.5 ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE |
Endotoxin | Less than 1 EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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