Biotinylated Human IL-2 R alpha/CD25 Protein (CD5-HM425B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-CD25 Antibody, hFc Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2 R alpha, His Tag with the EC50 of 11.9ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Glu22-Cys213

Catalog: CD5-HM425B

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Product Description Recombinant Biotinylated Human IL-2 R alpha/CD25 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu22-Cys213.
Product Category Recombinant Protein
Target IL-2 R alpha/CD25
Synonyms CD25; IDDM10; IL-2 R alpha; IL2R; IL2RA; p55; TCGFR
Gene/Protein ID P01589
Species Human
Exact Sequence Glu22-Cys213
Molecular Weight The protein has a predicted MW of 24.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-CD25 Antibody, hFc Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2 R alpha, His Tag with the EC50 of 11.9ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-CD25 Antibody, hFc Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2 R alpha, His Tag with the EC50 of 11.9ng/ml determined by ELISA.
The purity of Biotinylated Human IL-2 R alpha is greater than 95% as determined by SEC-HPLC.
Biotinylated Human IL-2 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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