Biotinylated Human IL-2 R alpha/CD25 Protein (CD5-HM425B)
Catalog: CD5-HM425B
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| Product Description | Recombinant Biotinylated Human IL-2 R alpha/CD25 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu22-Cys213. |
Product Background |
Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells. |
Product Category |
Recombinant Protein |
Target |
IL-2 R alpha/CD25 |
| Synonyms | CD25; IDDM10; IL-2 R alpha; IL2R; IL2RA; p55; TCGFR |
Gene/Protein ID |
P01589 |
Species |
Human |
Exact Sequence |
Glu22-Cys213 |
| Molecular Weight | The protein has a predicted MW of 24.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Anti-CD25 Antibody, hFc Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2 R alpha, His Tag with the EC50 of 11.9ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Anti-CD25 Antibody, hFc Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2 R alpha, His Tag with the EC50 of 11.9ng/ml determined by ELISA. |
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The purity of Biotinylated Human IL-2 R alpha is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human IL-2 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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