Name: Biotinylated Human IL-2 R alpha/CD25 Protein (CD5-HM425B)
Brand: KactusBio
SKU: CD5-HM425B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human IL-2 R alpha/CD25 Protein (CD5-HM425B)

Immobilized Biotinylated Human IL-2 R alpha, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 3.9 ng/ml determined by ELISA (QC Test).

Biotinylated Human IL-2 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human IL-2 R alpha is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human IL-2 R alpha, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 3.9 ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Glu22-Cys213

Catalog #CD5-HM425B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human IL-2 R alpha/CD25 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu22-Cys213.
Product Background	Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Product Category	Recombinant Protein / Antibody discovery

Protein	IL-2 R alpha/CD25
Synonyms	CD25; IDDM10; IL-2 R alpha; IL2R; IL2RA; p55; TCGFR
Accession	P01589
Species	Human
Biotinylated	yes
Amino Acid Range	Glu22-Cys213
Molecular Weight	The protein has a predicted MW of 24.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human IL-2 R alpha, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 3.9 ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human IL-2 R alpha, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 3.9 ng/ml determined by ELISA (QC Test).
	Biotinylated Human IL-2 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human IL-2 R alpha is greater than 95% as determined by SEC-HPLC.