Biotinylated Human IL-2 R alpha/CD25 Protein (CD5-HM425B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human IL-2 R alpha, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 30.8ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Glu22-Cys213

Catalog: CD5-HM425B

$657.00
Size:

Order now get it on

Product Description Recombinant Biotinylated Human IL-2 R alpha/CD25 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Glu22-Cys213.
Product Category Recombinant Protein
Target IL-2 R alpha/CD25
Synonyms CD25; IDDM10; IL-2 R alpha; IL2R; IL2RA; p55; TCGFR
Gene/Protein ID P01589
Species Human
Exact Sequence Glu22-Cys213
Molecular Weight The protein has a predicted MW of 24.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Human IL-2 R alpha, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 30.8ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human IL-2 R alpha, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 30.8ng/ml determined by ELISA.
The purity of Biotinylated Human IL-2 R alpha is greater than 95% as determined by SEC-HPLC.
Biotinylated Human IL-2 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

You may also be interested in