Biotinylated Human IL-3 R alpha/CD123 Protein (IL3-HM1RAB)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-IL-3 R alpha Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-3 R alpha, His Tag with the EC50 of 9.9ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Thr19-Arg305

Catalog: IL3-HM1RAB

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Product Description Recombinant Biotinylated Human IL-3 R alpha/CD123 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Thr19-Arg305.
Product Category Recombinant Protein
Target IL-3 R alpha/CD123
Gene/Protein ID P26951-1
Species Human
Exact Sequence Thr19-Arg305
Molecular Weight The protein has a predicted MW of 35.99 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-IL-3 R alpha Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-3 R alpha, His Tag with the EC50 of 9.9ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-IL-3 R alpha Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-3 R alpha, His Tag with the EC50 of 9.9ng/ml determined by ELISA.
The purity of Biotinylated Human IL-3 R alpha is greater than 95% as determined by SEC-HPLC.
Biotinylated Human IL-3 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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