ELISA binding curve showing biotinylated human IL-3 R alpha His-tag interaction with anti-IL-3 R alpha hFc-tagged antibody.
Biotinylated Human IL-3 R alpha/CD123 Protein (IL3-HM1RAB)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human IL-3 R alpha, His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-IL-3 R alpha Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Thr19-Arg305
Catalog #IL3-HM1RAB
Regular price $577.50
/
Size

Order now, ships by:

Biotinylated Human IL-3 R alpha/CD123 Protein (IL3-HM1RAB) Summary

Product Description

Recombinant Biotinylated Human IL-3 R alpha/CD123 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Thr19-Arg305.

Product Background

Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 5 subfamily.The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias.

Product Category

Recombinant Protein / Antibody discovery / CAR-T targets

Biotinylated Human IL-3 R alpha/CD123 Protein (IL3-HM1RAB) Specifications/Details

Protein
IL-3 R alpha/CD123
Synonyms
CD123; IL-3R subunit alpha; IL3R; IL3RA; IL-3Ra; IL3RAY; IL3RX; IL3RY; MGC34174; IL-3 R alpha
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Thr19-Arg305
Molecular Weight
The protein has a predicted MW of 35.99 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human IL-3 R alpha, His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-IL-3 R alpha Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human IL-3 R alpha, His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-IL-3 R alpha Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA. Immobilized Biotinylated Human IL-3 R alpha, His-Avi Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-IL-3 R alpha Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA.
Biotinylated Human IL-3 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human IL-3 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-3 R alpha is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human IL-3 R alpha is greater than 95% as determined by SEC-HPLC.
Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Recently viewed