Biotinylated Human LILRB1/CD85j/ILT2 Protein (LIL-HM4B1B)
Biotinylated Human LILRB1/CD85j/ILT2 Protein (LIL-HM4B1B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human LILRB1, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly24-His458
Catalog #LIL-HM4B1B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human LILRB1/CD85j/ILT2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly24-His458.
Product Background
LILRB1, also known as CD85j and ILT2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family. Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs.LILRB1 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response.
Product Category
Recombinant Protein
Target
LILRB1/CD85j/ILT2
Synonyms
ILT2; ILT-2; ILT2FLJ37515; LILRB1; LIR1; MIR7; CD85J; XXbac-BCX85G21.4
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Gly24-His458
Molecular Weight
The protein has a predicted MW of 50 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human LILRB1, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human LILRB1, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA.
Biotinylated Human LILRB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of-Biotinylated Human LILRB1 is greater than 95% as determined by SEC-HPLC.

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