Name: Biotinylated Human LILRB1/CD85j/ILT2 Protein (LIL-HM4B1B)
Brand: KactusBio
SKU: LIL-HM4B1B-100UG
Price: 577.5 USD
Availability: InStock

ELISA graph showing how strongly biotinylated human LILRB1 binds to its antibody. As the protein amount increases, the signal rises and levels off.

Biotinylated Human LILRB1/CD85j/ILT2 Protein (LIL-HM4B1B)

Immobilized Biotinylated Human LILRB1, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA (QC Test).

Biotinylated Human LILRB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of-Biotinylated Human LILRB1 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human LILRB1, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Gly24-His458

Catalog #LIL-HM4B1B

Size

100UG

500UG

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Biotinylated Human LILRB1/CD85j/ILT2 Protein (LIL-HM4B1B) Summary

Product Description	Recombinant Biotinylated Human LILRB1/CD85j/ILT2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly24-His458.
Product Background	LILRB1, also known as CD85j and ILT2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family. Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs.LILRB1 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Biotinylated Human LILRB1/CD85j/ILT2 Protein (LIL-HM4B1B) Specifications/Details

Protein	LILRB1/CD85j/ILT2
Synonyms	ILT2; ILT-2; ILT2FLJ37515; LILRB1; LIR1; MIR7; CD85J; XXbac-BCX85G21.4
Accession	Q8NHL6-1
Species	Human
Biotinylated	yes
Amino Acid Range	Gly24-His458
Molecular Weight	The protein has a predicted MW of 50 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human LILRB1, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human LILRB1, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA (QC Test).
	Biotinylated Human LILRB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of-Biotinylated Human LILRB1 is greater than 95% as determined by SEC-HPLC.