Biotinylated Human LRRC15/LIB Protein (LRR-HM415B)
Biotinylated Human LRRC15/LIB Protein (LRR-HM415B)
Biotinylated Human LRRC15/LIB Protein (LRR-HM415B)
Biotinylated Human LRRC15/LIB Protein (LRR-HM415B)
  • Express System: CHO
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Anti-LRRC15 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human LRRC15, His Tag with the EC50 of 35.6ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Tyr22-Gly538
Catalog #LRR-HM415B
Regular price $577.50
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Product Description

Recombinant Biotinylated Human LRRC15/LIB Protein is expressed from CHO with His tag and Avi tag at the C-Terminus.It contains Tyr22-Gly538.

Product Background

The chimeric protein binds in vitro and in vivo to a specific element upstream of LRRC15, leading to dramatic transcriptional activation.- LRRC15 encodes a leucine-rich transmembrane protein, present at the leading edge of migrating cells, the expression of which in normal tissues is restricted to the invasive cytotrophoblast layer of the placenta; small interfering (siRNA)-mediated suppression of LRRC15 expression in breast cancer cells leads to abrogation of invasiveness in vitro.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

LRRC15/LIB

Synonyms

LIB; LRRC15

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Tyr22-Gly538

Molecular Weight

The protein has a predicted MW of 60.7 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Anti-LRRC15 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human LRRC15, His Tag with the EC50 of 35.6ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His-Avi

Expression System

CHO

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Anti-LRRC15 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human LRRC15, His Tag with the EC50 of 35.6ng/ml determined by ELISA (QC Test). Immobilized Anti-LRRC15 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human LRRC15, His Tag with the EC50 of 35.6ng/ml determined by ELISA (QC Test).
Biotinylated Human LRRC15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human LRRC15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human LRRC15 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human LRRC15 is greater than 95% as determined by SEC-HPLC.

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