Biotinylated Human Mature TGF beta 1 Protein (TG1-HM10MB)
Catalog: TG1-HM10MB
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| Product Description | Recombinant Biotinylated Human Mature TGF beta 1 Protein is expressed from Expi293 with Avi tag at the C-terminal.It contains Ala279-Ser390. |
| Product Category | Recombinant Protein |
| Target | Mature TGF beta 1 |
| Synonyms | CEDLAP; DPD1; TGF beta1; TGFB; TGFB1; TGFbeta; TGF-beta-1 |
| Gene/Protein ID | P01137 |
| Species | Human |
| Exact Sequence | Ala279-Ser390 |
| Molecular Weight | The protein has a predicted MW of 13.2 kDa. Due to glycosylation, the protein migrates to 15-17 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human TGF-beta RII, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Mature TGF beta 1, Avi Tag with the EC50 of 10.0ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in 50mM Glycine, 150mM NaCl (pH 2.5). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM Glycine, 150mM NaCl (pH 2.5). |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human TGF-beta RII, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Mature TGF beta 1, Avi Tag with the EC50 of 10.0ng/ml determined by ELISA. |
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Biotinylated Human Mature TGF beta 1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. |
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