Biotinylated Human Mature TGF beta 1 Protein (TG1-HM10MB)

  • Express System: Expi293
  • Product Tag: C-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized Human TGF-beta RII, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Mature TGF beta 1, Avi Tag with the EC50 of 10.0ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ala279-Ser390

Catalog: TG1-HM10MB

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Product Description Recombinant Biotinylated Human Mature TGF beta 1 Protein is expressed from Expi293 with Avi tag at the C-terminal.; It contains Ala279-Ser390.
Product Category Recombinant Protein
Target Mature TGF beta 1
Synonyms CEDLAP; DPD1; TGF beta1; TGFB; TGFB1; TGFbeta; TGF-beta-1
Gene/Protein ID P01137
Species Human
Exact Sequence Ala279-Ser390
Molecular Weight The protein has a predicted MW of 13.2 kDa. Due to glycosylation, the protein migrates to 15-17 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human TGF-beta RII, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Mature TGF beta 1, Avi Tag with the EC50 of 10.0ng/ml determined by ELISA. See testing image for detail.
Product Tag C-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in 50mM glycine, 150mM NaCl (pH 2.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in 50mM glycine, 150mM NaCl (pH 2.5).
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human TGF-beta RII, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Mature TGF beta 1, Avi Tag with the EC50 of 10.0ng/ml determined by ELISA.
Biotinylated Human Mature TGF beta 1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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