Biotinylated Human Mature TGF beta 1 Protein (TG1-HM10MB)
Biotinylated Human Mature TGF beta 1 Protein (TG1-HM10MB)
  • Express System: HEK293
  • Product Tag: C-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human TGF-beta RII, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Mature TGF beta 1, Avi Tag with the EC50 of 9.9ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala279-Ser390
Catalog #TG1-HM10MB
Regular price $600.00
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Product Description

Recombinant Biotinylated Human Mature TGF beta 1 Protein is expressed from HEK293 with Avi tag at the C-Terminus.It contains Ala279-Ser390.

Product Background

TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition.Transforming growth factor beta-1 is multifunctional protein that regulates the growth and differentiation of various cell types and is involved in various processes, such as normal development, immune function, microglia function and responses to neurodegeneration.

Product Category

Recombinant Protein / Antibody discovery

Protein

Mature TGF beta 1

Synonyms

CEDLAP; DPD1; TGF beta1; TGFB; TGFB1; TGFbeta; TGF-beta-1; TGF β1; TGFβ; TGF-β-1

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Ala279-Ser390

Molecular Weight

The protein has a predicted MW of 13.2 kDa. Due to glycosylation, the protein migrates to 15-18 kDa under reduced (R) condition and 26-30 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.

Activity

Immobilized Human TGF-beta RII, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Mature TGF beta 1, Avi Tag with the EC50 of 9.9ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in 50mM Glycine, 150mM NaCl (pH 2.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 4mM HCl.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human TGF-beta RII, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Mature TGF beta 1, Avi Tag with the EC50 of 9.9ng/ml determined by ELISA. Immobilized Human TGF-beta RII, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Mature TGF beta 1, Avi Tag with the EC50 of 9.9ng/ml determined by ELISA.
Biotinylated Human Mature TGF beta 1 on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%. Biotinylated Human Mature TGF beta 1 on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.

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