Biotinylated Human MSLN/Mesothelin Protein (MSL-HM480B)
Catalog: MSL-HM480B
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| Product Description | Recombinant Biotinylated Human MSLN/Mesothelin Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu296-Gly580. |
Product Background |
Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally. |
Product Category |
Recombinant Protein |
Target |
MSLN/Mesothelin |
| Synonyms | MSLN; CAK1; Mesothelin; MPF; MPFSMRP; SMR |
Gene/Protein ID |
Q13421-2 |
Species |
Human |
Exact Sequence |
Glu296-Gly580 |
| Molecular Weight | The protein has a predicted MW of 35.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Anti-MSLN Antibody at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human MSLN, His Tag with the EC50 of 20.9ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Anti-MSLN Antibody at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human MSLN, His Tag with the EC50 of 20.9ng/ml determined by ELISA. |
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The purity of Biotinylated Human MSLN was greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human MSLN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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