Biotinylated Human MSLN/Mesothelin Protein (MSL-HM4D1B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human MSLN, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Met487-Ser598

Catalog: MSL-HM4D1B

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Product Description Recombinant Biotinylated Human MSLN/Mesothelin Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Met487-Ser598.

Product Background

 Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.

Product Category

 Recombinant Protein

Target

 MSLN/Mesothelin
Accession

Species

 Human

Amino Acid Range

 Met487-Ser598
Molecular Weight The protein has a predicted MW of 15.31 kDa. Due to glycosylation, the protein migrates to 20-40 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Biotinylated Human MSLN, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA. See testing image for detail.

Product Tag

 C-His-Avi

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human MSLN, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA.
The purity of Biotinylated Human MSLN is greater than 95% as determined by SEC-HPLC.
Biotinylated Human MSLN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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