Biotinylated Human MSLN/Mesothelin Protein (MSL-HM4D1B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human MSLN, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Met487-Ser598
Catalog #MSL-HM4D1B
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Product Description
 Recombinant Biotinylated Human MSLN/Mesothelin Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Met487-Ser598.
Product Background
 Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.
Product Category
 Recombinant Protein
Target
 MSLN/Mesothelin
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Met487-Ser598
Molecular Weight
 The protein has a predicted MW of 15.31 kDa. Due to glycosylation, the protein migrates to 20-40 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human MSLN, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human MSLN, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA.
Biotinylated Human MSLN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human MSLN is greater than 95% as determined by SEC-HPLC.
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