Biotinylated Human MSLN/Mesothelin Protein (MSL-HM580B)

  • Express System: Expi293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-MSLN Antibody at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human MSLN, hFc Tag with the EC50 of 13.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Glu296-Asp580

Catalog: MSL-HM580B

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Product Description Recombinant Biotinylated Human MSLN/Mesothelin Protein is expressed from Expi293 with hFc tag and Avi tag at the C-terminal.; It contains Glu296-Asp580.
Product Category Recombinant Protein
Target MSLN/Mesothelin
Synonyms MSLN; CAK1; Mesothelin; MPF; MPFSMRP; SMR
Gene/Protein ID Q13421-2
Species Human
Exact Sequence Glu296-Asp580
Molecular Weight The protein has a predicted MW of 61.1 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-MSLN Antibody at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human MSLN, hFc Tag with the EC50 of 13.7ng/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-MSLN Antibody at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human MSLN, hFc Tag with the EC50 of 13.7ng/ml determined by ELISA.
Use Biotinylated Human MSLN-hFc-Avi protein to detect the expression rate of Anti-MSLN-CAR positive cell. 293T cells and anti-MSLN CAR-293T cells were incubated with Biotinylated human MSLN-hFc-Avi Tag. Non-transfected 293T cells and Fc-labeled protein control were used as negative control. SA-FITC was used to evaluate the binding activity of Biotinylated Human MSLN.
The purity of Biotinylated Human MSLN is greater than 95% as determined by SEC-HPLC.
Biotinylated Human MSLN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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