Product Description |
Recombinant Biotinylated Human NCAM-1/CD56 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Leu20-Gly718. |
Product Background |
Neural Cell Adhesion Molecule 1 (NCAM-1), a multifunctional member of the immunoglobulin superfamily, is expressed on the surface of neurons, glia, skeletal muscle, and natural killer cells. NCAM-1 has been implicated as having a role in cell-cell adhesion, involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. |
Product Category |
Recombinant Protein |
Target |
NCAM-1/CD56 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Leu20-Gly718 |
Molecular Weight |
The protein has a predicted MW of 80.29 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human NCAM-1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NCAM-1 Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Biotinylated Human NCAM-1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NCAM-1 Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA. |
|
|
Biotinylated Human NCAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Biotinylated Human NCAM-1 is greater than 95% as determined by SEC-HPLC. |
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