Biotinylated Human NCAM-1/CD56 Protein (CAM-HM456B)
Biotinylated Human NCAM-1/CD56 Protein (CAM-HM456B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human NCAM-1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NCAM-1 Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Leu20-Gly718
Catalog #CAM-HM456B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human NCAM-1/CD56 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Leu20-Gly718.
Product Background
Neural Cell Adhesion Molecule 1 (NCAM-1), a multifunctional member of the immunoglobulin superfamily, is expressed on the surface of neurons, glia, skeletal muscle, and natural killer cells. NCAM-1 has been implicated as having a role in cell-cell adhesion, involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance.
Product Category
Recombinant Protein
Target
NCAM-1/CD56
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Leu20-Gly718
Molecular Weight
The protein has a predicted MW of 80.29 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human NCAM-1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NCAM-1 Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human NCAM-1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NCAM-1 Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA.
Biotinylated Human NCAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human NCAM-1 is greater than 95% as determined by SEC-HPLC.

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