Biotinylated Human NCAM-1/CD56 Protein (CAM-HM456B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human NCAM-1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NCAM-1 Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Leu20-Gly718

Catalog: CAM-HM456B

$525.00
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Product Description Recombinant Biotinylated Human NCAM-1/CD56 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Leu20-Gly718.

Product Background

Neural Cell Adhesion Molecule 1 (NCAM-1), a multifunctional member of the immunoglobulin superfamily, is expressed on the surface of neurons, glia, skeletal muscle, and natural killer cells. NCAM-1 has been implicated as having a role in cell-cell adhesion, involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance.

Product Category

Recombinant Protein

Target

NCAM-1/CD56
Accession

Species

Human

Amino Acid Range

Leu20-Gly718
Molecular Weight The protein has a predicted MW of 80.29 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Biotinylated Human NCAM-1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NCAM-1 Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human NCAM-1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NCAM-1 Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA.
The purity of Biotinylated Human NCAM-1 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human NCAM-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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