Biotinylated Human Nectin-4 Protein IgV Domain (NEC-HM414B)
Catalog: NEC-HM414B
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| Product Description | Recombinant Biotinylated Human Nectin-4 Protein IgV Domain is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly32-Leu146. |
Product Background |
Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus. |
Product Category |
Recombinant Protein |
Target |
Nectin-4 IgV Domain |
| Synonyms | EDSS1; LNIR; Nectin4; PRR4; NECTIN4; PVRL4 |
Gene/Protein ID |
Q96NY8-1 |
Species |
Human |
Exact Sequence |
Gly32-Leu146 |
| Molecular Weight | The protein has a predicted MW of 15.8 kDa. Due to glycosylation, the protein migrates to 16-20 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Anti-Nectin-4 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Nectin-4 IgV Domain, His Tag with the EC50 of 30.9ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Anti-Nectin-4 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Nectin-4 IgV Domain, His Tag with the EC50 of 30.9ng/ml determined by ELISA. |
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The purity of Biotinylated Human Nectin-4 IgV Domain is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human Nectin-4 IgV Domain on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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